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| 土壤中放线菌参与反硝化可能性研究 | |
| 引用本文： | 葛灿,石竹南,颜志民,杨林章,王德建,尹斌,梅丽娟,朴哲,胡健,殷士学.土壤中放线菌参与反硝化可能性研究[J].土壤学报,2004,41(1):108-112. |
| 作者姓名： | 葛灿 石竹南 颜志民 杨林章 王德建 尹斌 梅丽娟 朴哲 胡健 殷士学 |
| 作者单位： | 1. 扬州大学农学院农学系,江苏扬州,225009 2. 扬州大学物理科学与技术学院,江苏扬州,225009 3. 扬州市环境监测总站,江苏扬州,225007 4. 中国科学院南京土壤研究所,南京,210008 |
| 基金项目： | 国家自然科学基金项目(30170029)、中国科学院南京土壤研究所土壤圈物质循环开放实验室基金课题、中国科学院知识创新工程项目(KZCX2-413-3-1)资助 |
| 摘 要： | 对土壤中放线菌参与土壤反硝化可能性进行了初步研究。从土壤 10 - 5 稀释液中共获得 2 3个放线菌单菌落 ,初步鉴定属于 10个不同类型的菌株 ,其中 9个属于Streptomyces (链霉菌属 ) ,1个属于Actino madura (马杜拉属 )。 10个菌株在纯培养条件下都能将NO-3 还原成N2 O ,表明该土壤中具有反硝化能力的放线菌比例很高。测得其中 3个链霉菌菌株的N2 O产出速率为N 1 2～ 187 7μgg- 1min- 1(细胞干重基数 )。N2 O形成与放线菌生物量成正比。多数链霉菌菌株的N2 O产出受C2 H2 抑制。将 3个链霉菌菌株接种到灭菌土壤中厌气培养 ,均能测到N2 O形成 ,表明链霉菌可以利用土壤中原有碳源作为电子供体还原NO-3 。经历厌氧胁迫 2 1d以后 ,测试的 3株链霉菌菌株至少能保留一部分反硝化能力 |
| 关 键 词： | 放线菌 反硝化 土壤微生物 |
| 收稿时间： | 2003/6/23 0:00:00 |
| 修稿时间： | 9/3/2003 12:00:00 AM |
PARTICIPANT POSSIBILITY OF ACTINOMYCETES IN SOIL DENITRIFICATION |
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| Ge Can,Shi Zhunan,Yan Zhimin,Yang Linzhang,Wang Dejian,Yin Bin,Mei Lijuan,Piao Zhe,Hu Jian and Yin Shixue.PARTICIPANT POSSIBILITY OF ACTINOMYCETES IN SOIL DENITRIFICATION[J].Acta Pedologica Sinica,2004,41(1):108-112. | |
| Authors: | Ge Can Shi Zhunan Yan Zhimin Yang Linzhang Wang Dejian Yin Bin Mei Lijuan Piao Zhe Hu Jian and Yin Shixue |
| Affiliation: | Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou, Jiangsu 225009, China;College of Physics Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China;General Station of Environmental Monitoring, Yangzhou, Jiangsu 225007, China;Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou, Jiangsu 225009, China;Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou, Jiangsu 225009, China;Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou, Jiangsu 225009, China;Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou, Jiangsu 225009, China |
| Abstract: | Twenty three single colonies of actinomycetes were isolated from a soil sample collected from Changshu Agroecological. Experimental Station,Institute of Soil Science,Chinese Academy of Sciences Among 10 strains distinguished from each other by morphological properties,9 were further identified as Streptomyces sps. All strains showed capability of reducing nitrate into N2O under anaerobic pure culture conditions. Three strains were selected and denitrification rates were measured. Nitrous oxide product ion rate of these strains ranged from N 1.2 to 187.7 g g-1 min-1. The strains were shown not only to grow well but also to be able to reduce nitrate to N2O in sterile soil,indicating that these strains were able to use soil organic carbon as electron donor for nitrate reduction. Acetylene showed inhibitory effect on N2O production of most strains isolated. Stressed under continuous strict anaerobic condition,strains of Streptomyces sps still showed considerable denitrification capacities. |
| Keywords: | Actinomycetes Denitrification Soil microorganisms |
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