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生物炭对设施退化土壤氮相关功能微生物群落丰度的影响
引用本文:王晓辉,郭光霞,郑瑞伦,钟 敏,朱永官.生物炭对设施退化土壤氮相关功能微生物群落丰度的影响[J].土壤学报,2013,50(3):624-631.
作者姓名:王晓辉  郭光霞  郑瑞伦  钟 敏  朱永官
作者单位:1. 中国科学院生态环境研究中心,北京100085;中国科学院大学,北京100049
2. 中国科学院生态环境研究中心,北京,100085
3. 中国科学院生态环境研究中心,北京100085;中国科学院城市环境研究所,厦门361021
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
摘    要:设施栽培土壤在人工调控下进行生产,长期处在高温高湿、无降水淋洗、高复种指数、持续大量施肥等特殊环境条件下,它的物理结构和生化性状产生很大变化.菜农因传统施肥经验而形成的盲目大量施肥行为造成氮肥的过量摄入,从而愈发加重了土壤表层养分富集、酸化板结、养分失调等,继而导致蔬菜品质严重下降,在部分种植年限较长的大棚已不能进行蔬菜生产,严重制约了各地区设施农业的可持续发展1].而长期过量的施用氮肥还会使设施土壤中硝态氮含量升高,减少土壤中的硝化微生物菌群的丰度,提高反硝化微生物的活性,促进N2O的排放通量2].因此,为了保证设施栽培产业能够健康、可持续地发展,使蔬菜生产向高产、高效、优质的方向发展,对设施栽培土壤氮循环的研究已迫在眉睫.

关 键 词:生物炭  设施栽培土壤  硝化作用  反硝化作用
收稿时间:9/18/2012 5:27:03 PM
修稿时间:12/4/2012 9:39:46 AM

Effect of biochar on abundance of N-related functional microbial communities in degraded greenhouse soil
Wang Xiaohui,Guo Guangxi,Zheng Ruilun,Zhong Min and Zhu Yongguan.Effect of biochar on abundance of N-related functional microbial communities in degraded greenhouse soil[J].Acta Pedologica Sinica,2013,50(3):624-631.
Authors:Wang Xiaohui  Guo Guangxi  Zheng Ruilun  Zhong Min and Zhu Yongguan
Affiliation:Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences;University of Chinese Academy of Sciences,Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences,Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences,Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences,Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences; Institute of Urban Environment, Chinese Academy of Sciences
Abstract:The effect of biochar on abundances of nitrifying and denitrifying communities and the concentrations of nitrite and nitrate in rapes were investigated by amending 5%(wt/wt) biochar (rice straw biochar and sludge biochar) to a degraded greenhouse soil under vegetable cultivation for over 15 years. Results show that only the rice straw biochar significantly improved soil potential nitrification rates and increased the abundance of Bacterial amoA gene and nirK gene in the soil. In addition, the rice straw biochar also reduced the concentrations of nitrate and nitrite in rapes, whereas the sludge biochar significantly decreased the concentrations of ammonium in rapes. This study, for the first time, examined the effect of biochar on abundances of nitrifying and denitrifying communities in the degraded greenhouse soil. These findings provided mechanistic information on how biochar affects soil N transformations, and the way to remediate the degraded greenhouse soil and to reduce the concentrations of nitrate and nitrite in vegetables.
Keywords:Biochar  Degraded greenhouse soil  Nitrification  Denitrification
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