| 番茄SlJAZ11的表达分析及互作蛋白的筛选与验证 |
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| 引用本文： | 高苗苗,郭鑫,朱寿松,黄思源,李建君,庞春娜,陈银华,于晓惠.番茄SlJAZ11的表达分析及互作蛋白的筛选与验证[J].核农学报,2023,37(3):483-494. |
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| 作者姓名： | 高苗苗 郭鑫 朱寿松 黄思源 李建君 庞春娜 陈银华 于晓惠 |
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| 作者单位： | 1.海南大学，海南省热带生物资源可持续利用重点实验室，海南 海口 5702282.海南大学热带作物学院，海南 海口 5702283.中国热带农业科学院热带生物技术研究所，海南 海口 571101 |
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| 基金项目： | 海南省自然科学基金高层次人才项目(320RC480);国家自然科学基金(31960526) |
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| 摘 要： | 茉莉酸（JA）参与调节植物生长、发育和防御等多种重要生物过程，其中JAZ蛋白作为抑制子，在茉莉酸介导的生物和非生物胁迫响应过程中起到重要作用。为研究SlJAZ11的功能，本研究分析了水杨酸（SA）、茉莉酸等激素以及病原菌侵染处理后SlJAZ11的表达模式。结果表明，SlJAZ11能够响应水杨酸、茉莉酸及丁香假单胞菌（Pst DC3000）的诱导。通过酵母双杂交筛选番茄cDNA文库，获得了14个SlJAZ11潜在的互作蛋白。进一步采用酵母双杂交点对点验证和双分子荧光互补技术（BiFC）对候选的SlENT、SlOOLG与SlJAZ11间的互作关系进行验证，发现SlENT和SlJAZ11存在互作。对SlENT的表达模式进行分析，发现其表达被SA和Pst DC3000显著抑制，而MeJA能显著诱导其表达，表明SlJAZ11可能通过与SlENT互作来调控JA介导的番茄抗病性。本研究可为阐明SlJAZ11功能与作用机制奠定良好的分子基础。
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| 关 键 词： | 番茄 茉莉酸 SlJAZ11 酵母双杂交 SlENT |
| 收稿时间： | 2022-06-17 |
Expression Analysis of Tomato SlJAZ11 and Screening and Validation of Its Interacting Proteins |
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| GAO Miaomiao,GUO Xin,ZHU Shousong,HUANG Siyuan,LI Jianjun,PANG Chunna,CHEN Yinhua,YU Xiaohui.Expression Analysis of Tomato SlJAZ11 and Screening and Validation of Its Interacting Proteins[J].Acta Agriculturae Nucleatae Sinica,2023,37(3):483-494. |
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| Authors: | GAO Miaomiao GUO Xin ZHU Shousong HUANG Siyuan LI Jianjun PANG Chunna CHEN Yinhua YU Xiaohui |
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| Affiliation: | 1.Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources，Hainan University，Haikou，Hainan 5702282.College of Tropical Crops，Hainan University，Haikou，Hainan 5702283.Institute of Tropical Biotechnology，Chinese Academy of Tropical Agricultural Sciences，Haikou，Hainan 571101 |
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| Abstract: | Jasmonic acid （JA） is involved in regulating various important biological processes such as plant growth， development and defense， in which JAZ proteins play an important role as the repressor in jasmonic acid-mediated biotic and abiotic stress response processes. To investigate the function of SlJAZ11， this study analyzed the expression pattern of SlJAZ11 after treatment with hormones such as salicylic acid （SA） and jasmonic acid （JA） as well as pathogenic bacteria infection， and the results showed that SlJAZ11 was able to respond to the induction of salicylic acid， jasmonic acid and Pseudomonas syringae pv. tomato （Pst DC3000）. A tomato cDNA library was screened by yeast two-hybrid assay， and 14 potential interacting proteins of SlJAZ11 were obtained. The interactions between the candidate SlENT， SlOOLG and SlJAZ11 were further verified by yeast two-hybrid point-to-point assay and bimolecular fluorescence complementation technique （BiFC）， and the interactions between SlENT and SlJAZ11 were confirmed. Analysis of the expression pattern of SlENT revealed that its expression was significantly inhibited by SA and Pst DC3000， while MeJA significantly induced its expression， indicating that SlJAZ11 may regulate JA-mediated disease resistance in tomato by interacting with SlENT. This study may lay a good molecular foundation for elucidating the function and mechanism of SlJAZ11. |
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| Keywords: | tomato jasmonic acid SlJAZ11 yeast two-hybrid SlENT |
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