| 宛氏拟青霉提取物诱导小白菜抗低温胁迫的作用机理 |
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| 引用本文： | 王庆彬,刘治国,彭春娥,孟慧,赵红玲,王洪凤,张民.宛氏拟青霉提取物诱导小白菜抗低温胁迫的作用机理[J].核农学报,2022,36(2):473-480. |
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| 作者姓名： | 王庆彬 刘治国 彭春娥 孟慧 赵红玲 王洪凤 张民 |
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| 作者单位： | 1山东农业大学资源与环境学院/土肥资源高效利用国家工程实验室,山东 泰安 2710182山东蓬勃生物科技有限公司,山东 泰安 2710183山东农业大学生命科学学院/作物生物学国家重点实验室,山东 泰安 2710184山东蓬创农业技术开发有限公司,山东 泰安 271018 |
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| 基金项目： | 国家重点研发计划项目(2017YFD0200706)； |
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| 摘 要： | 为了揭示宛氏拟青霉提取物(ZNC)诱导小白菜抗低温胁迫的作用机理,本研究以黄秧小白菜为试验材料,探究叶喷不同浓度(20、30、40 ng·mL-1)ZNC对低温下小白菜生长的影响。结果表明,低温下,与清水对照相比,喷施不同浓度的ZNC均可显著提高小白菜的SPAD值、株高和地上部鲜重。ZNC还提高了小白菜叶片内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,并降低了过氧化氢(H2O2)和丙二醛(MDA)的积累量。此外,ZNC不仅可以通过上调小白菜抗寒基因BrCBF和BrCOR14的表达水平增加渗透调节物质,如可溶性蛋白和脯氨酸的含量,还可以增加赤霉素(GA)和吲哚乙酸(IAA)含量,降低脱落酸(ABA)含量。上述结果表明,ZNC可通过降低低温对小白菜细胞膜造成的氧化损伤,增加渗透调节物质积累以保持细胞结构稳定,提高(GA+IAA)/ABA比值来促进小白菜生长,最终增强小白菜对低温胁迫的抗性。本研究为降低低温对蔬菜的危害提供了有效手段。
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| 关 键 词： | 宛氏拟青霉提取物(ZNC) 小白菜 低温胁迫 抗氧化性 植物激素 |
| 收稿时间： | 2021-03-12 |
Mechanism of Paecilomyces variotii Extract in Inducing Pakchoi Resistance to Low-Temperature Stress |
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| WANG Qingbin,LIU Zhiguo,PENG Chun'e,MENG Hui,ZHAO Hongling,WANG Hongfeng,ZHANG Min.Mechanism of Paecilomyces variotii Extract in Inducing Pakchoi Resistance to Low-Temperature Stress[J].Acta Agriculturae Nucleatae Sinica,2022,36(2):473-480. |
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| Authors: | WANG Qingbin LIU Zhiguo PENG Chun'e MENG Hui ZHAO Hongling WANG Hongfeng ZHANG Min |
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| Affiliation: | (National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources/College of Resources and Environment,Shandong Agricultural University,Tai'an,Shandong 271018;Shandong Pengbo Biotechnology Co.,LTD,Tai'an,Shandong 271018;State Key Laboratory of Crop Biology/College of Life Sciences,Shandong Agricultural University,Tai'an,Shandong 271008;Shandong Pengchuang Agricultural Technology Development Co.,LTD,Tai'an,Shandong 271018) |
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| Abstract: | In this study,to reveal the mechanism of Paecilomyces variotii extract(ZNC)induced pakchoi(Brassica campestris L.)resistance to low-temperature stress,a pakchoi cultivar named Yellow seedlings was used to study the effects on pakchoi growth treated with different concentrations of ZNC(20,30 and 40 ng·mL-1).The results showed that all the concentrations of ZNC increased SPAD value,plant height and fresh weight of pakchoi significantly at low temperature compared with water treatment.The enzyme activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)in pakchoi leaves were also improved,while hydrogen peroxide(H2O2)and malondialdehyde(MDA)accumulation were decreased by using ZNC.Besides,ZNC could increase the contents of soluble protein and proline by up-regulating the expression level of cold-resistant related genes BrCBF and BrCOR14 in pakchoi.The contents of GA and IAA in pakchoi were also significantly increased after ZNC treatment,while the content of ABA was decreased.These results indicated that ZNC enhanced pakchoi resistance to low-temperature stress through reducing the cell membrane oxidative damage,keeping the cell structure stable by increasing osmotic adjustment substances accumulation,and promoting the growth of the pakchoi by improving the ratio of(GA+IAA)/ABA.Hence,this study provides an effective means to reduce the harm of low temperature to pakchoi and other vegetables. |
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| Keywords: | Paecilomyces variotii extracts(ZNC) pakchoi chilling stress oxidation resistance phytohormone |
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