| E-cadherin在牦牛不同繁殖周期输卵管不同部位中的表达与定位 |
| |
| 引用本文： | 杨珊珊,何翃闳,刘敏清,赵凌,王靖雷,余四九,蔡得琪,潘阳阳.E-cadherin在牦牛不同繁殖周期输卵管不同部位中的表达与定位[J].核农学报,2022,36(1):57-65. |
| |
| 作者姓名： | 杨珊珊 何翃闳 刘敏清 赵凌 王靖雷 余四九 蔡得琪 潘阳阳 |
| |
| 作者单位： | 1甘肃农业大学动物医学院/甘肃省牛羊胚胎工程技术研究中心,甘肃 兰州 7300702甘肃省金昌市金川区农业农村局, 甘肃 金昌 737100 |
| |
| 基金项目： | 国家自然科学基金(31972760);甘肃省教育厅产业支撑引导项目(2019C-03);甘肃农业大学伏羲青年英才基金(Gaufx-02Y10);甘肃农业大学博士科研启动基金(GSAU-RCZX201701)。 |
| |
| 摘 要： | 为研究上皮钙粘蛋白(E-cadherin)在不同繁殖周期牦牛输卵管不同部位中的表达差异,本研究采集卵泡期、黄体期和妊娠期无临床病理表现的母牦牛(3~6 岁)输卵管伞部、壶腹部和峡部的样品,将不同繁殖周期输卵管分别按照伞部、壶腹部和峡部分为9组,采用实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹法(western-blotting)检测E-cadherin基因和蛋白在不同时期牦牛输卵管不同部位中的表达情况,运用免疫组织化学法检测E-cadherin在不同时期牦牛输卵管不同部位中的定位。结果显示,在卵泡期,E-cadherin基因和蛋白在输卵管壶腹部的表达量最高,显著高于伞部和峡部;在黄体期,E-cadherin基因和蛋白在输卵管峡部的表达量最高,显著高于伞部和壶腹部;在妊娠期,E-cadherin基因在输卵管壶腹部的表达量最高,显著高于伞部和峡部,而E-cadherin蛋白在输卵管壶腹部的表达量最高,显著高于伞部。在输卵管伞部,E-cadherin基因在黄体期的表达量最高,显著高于卵泡期和妊娠期,而E-cadherin蛋白在卵泡期的表达量最高,显著高于黄体期和妊娠期;在输卵管壶腹部,E-cadherin基因和蛋白在妊娠期的表达量最高,显著高于卵泡期和黄体期;在输卵管峡部,E-cadherin基因和蛋白在黄体期的表达量最高,显著高于卵泡期和妊娠期。免疫组织化学结果显示,E-cadherin主要分布于输卵管伞部、壶腹部和峡部的纤毛细胞、分泌细胞、基细胞、肌层和浆液腺等部位。E-cadherin在牦牛繁殖周期输卵管不同部位中的均有表达,且其基因和蛋白的表达存在显著差异,说明E-cadherin可能参与了牦牛卵母细胞的生长和成熟、精子储存和获能、受精及早期胚胎发育等一系列生殖过程。本研究为进一步明确E-cadherin对牦牛繁殖性能的生物学作用提供了基础资料。
|
| 关 键 词： | 上皮钙粘蛋白 牦牛 输卵管 繁殖周期 表达 |
| 收稿时间： | 2020-11-13 |
Expression and Localization of E-cadherin in Different Parts of the Oviduct at Different Reproduction Cycles of Yak |
| |
| YANG Shanshan,HE Honghong,LIU Minqing,ZHAO Ling,WANG Jinglei,YU Sijiu,CAI Deqi,PAN Yangyang.Expression and Localization of E-cadherin in Different Parts of the Oviduct at Different Reproduction Cycles of Yak[J].Acta Agriculturae Nucleatae Sinica,2022,36(1):57-65. |
| |
| Authors: | YANG Shanshan HE Honghong LIU Minqing ZHAO Ling WANG Jinglei YU Sijiu CAI Deqi PAN Yangyang |
| |
| Affiliation: | 1College of Veterinary Medicine/Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Gansu Agricultural University, Lanzhou, Gansu 7300702Agriculture and Rural Bureau of Jinchuan District of Jinchang City of Gansu Province, Jinchang, Gansu 737100 |
| |
| Abstract: | The objective of this study was carried out to study the expression differences of E-cadherin in different parts of the oviduct at different reproduction cycles of yak,and to provide some important theoretical foundations for understanding the effect of E-cadherin on it′s reproductive performance.The samples of the tubal umbrella,ampulla,and isthmus of female yaks(3~6 years old)with no clinical pathological manifestations during follicular,luteal and pregnant phases were collected,the different reproduction cycles of yak are divided into 9 groups according to the umbrella,ampulla and isthmus.Real-time PCR(qRT-PCR)and western-blotting were used to detect the expression of E-cadherin gene and protein.The expression of E-cadherin protein was localized by immunohistochemistry.The results showed that during follicular phase,the expression of E-cadherin gene and protein in the ampulla of the oviduct was significantly higher than that in the umbrella and isthmus.During luteal phase,the expression of E-cadherin gene and protein in the isthmus of the oviduct was significantly higher than that in the umbrella and ampulla.During pregnant phase,the expression of E-cadherin gene in the ampulla of the oviduct was significantly higher than that in the umbrella and isthmus,and the expression of E-cadherin protein in the ampulla of the oviduct was significantly higher than that in the umbrella In the oviduct umbrella,the expression of E-cadherin gene in the luteal phase was significantly higher than that in the follicular phase and the pregnant phase,and the expression of E-cadherin protein in the follicular phase was significantly higher than that in the luteal phase and the pregnant phase.In the oviduct ampulla,the expression of E-cadherin gene and protein in the pregnant phase was significantly higher than that in the follicular phase and luteal phase.In the oviduct isthmus,the expression of E-cadherin gene and protein in the luteal phase was significantly higher than that in the follicular phase and pregnant phase.The results of immunohistochemistry showed that E-cadherin protein was positively expressed in ciliated cells,secretory cells,basal cells,muscular layers and serous glands of the oviduct umbrella,ampulla and isthmus.The results suggested that E-cadherin in different parts of the oviduct at reproduction cycles of the yak was located,and the expression of E-cadherin gene and protein was significantly different,which may be involved in a series of reproductive processes such as the growth and maturation of oocytes,sperm storage and capacitation,fertilization and early embryonic development of yak,and provided basic information for further clarifying of the biological effect of E-cadherin on yak′s reproductive performance. |
| |
| Keywords: | E-cadherin yak oviduct reproduction cycle expression |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《核农学报》浏览原始摘要信息 |
|
点击此处可从《核农学报》下载全文 |
|
