| 转Bt基因“克螟稻”秸秆还田对稻田厌氧微生物种群和酶活性的影响 |
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| 引用本文： | 徐晓宇,叶庆富,吴伟祥,闵航.转Bt基因“克螟稻”秸秆还田对稻田厌氧微生物种群和酶活性的影响[J].植物营养与肥料学报,2004,10(1):63-67. |
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| 作者姓名： | 徐晓宇 叶庆富 吴伟祥 闵航 |
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| 作者单位： | 1.浙江大学生命科学学院 浙江杭州310029; |
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| 摘 要： | 采用改良亨盖特技术,通过最大或然数法(mostprobablenumber,MPN法)及固体滚管法,系统研究了转Bt基因克螟稻秸秆对淹水条件下稻田土壤厌氧微生物种群的影响;同时采用比色法,研究其对土壤酶活性的影响。结果表明,克螟稻秸秆对水田土壤反硝化细菌和产甲烷细菌种群有显著的抑制作用(p0.05);对厌氧发酵细菌种群有显著的刺激作用(P0.05);而对厌氧固氮细菌种群有刺激作用但差异未达到显著水平(p0.05)。克螟稻秸秆还田在短期内可显著提高土壤纤维素酶、磷酸酶活性(p0.05),但对脱氢酶活性有明显抑制作用(p0.05)。
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| 关 键 词： | 克螟稻 秸秆还田 厌氧微生物 土壤酶活性 |
| 文章编号： | 1008-505X(2004)01-0063-05 |
| 修稿时间： | 2002年12月20 |
Effects of transgenic Bt rice straw on anaerobic microbial populations and enzyme activities in paddy soil |
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| XU Xiao-yu,YE Qing-fu,WU Wei-xiang,MIN Hang.Effects of transgenic Bt rice straw on anaerobic microbial populations and enzyme activities in paddy soil[J].Plant Nutrition and Fertilizer Science,2004,10(1):63-67. |
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| Authors: | XU Xiao-yu YE Qing-fu WU Wei-xiang MIN Hang |
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| Affiliation: | 1.College of Life Sci.;Zhejiang Univ.;Hangzhou 310029;China; |
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| Abstract: | The influences of transgenic Bt rice straw on anaerobic microbial populations and enzymatic activities in paddy soil were investigated by using the improved Hungate anaerobic technique, including anaerobic MPN (most probable number), roll tube and spectrophotometer methods. The results showed that the transgenic Bt rice straw could not only increase the number of hydrolytic-fermentative bacteria (P<0.05) and decrease obviously that of denitrifying and methanogenic bacteria (P<0.05), but also increase the population of anaerobic nitrogen-fixing bacteria significantly. The significant effect of transgenic Bt rice straw was observed on the activities of phosphatase and cellulase (P<0.05), however, the activity of dehydrogenase was seriously inhibited ((P<0.05)) in short time after returning transgenic rice straw into flooded soil. |
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| Keywords: | Kemingdao straw returning to the soil anaerobic microorganism soil enzyme activity |
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