| 硼对豌豆根尖细胞壁组分对铝吸附解吸的影响 |
| |
| 引用本文： | 冯英明,罗功荣,曲梅,玄祖迎,李学文,麦靖文,喻敏.硼对豌豆根尖细胞壁组分对铝吸附解吸的影响[J].植物营养与肥料学报,2022,28(10):1893-1900. |
| |
| 作者姓名： | 冯英明 罗功荣 曲梅 玄祖迎 李学文 麦靖文 喻敏 |
| |
| 作者单位： | 1.佛山科学技术学院国际膜生物学与环境研究中心 / 食品科学与工程学院园艺系，广东佛山 528200 |
| |
| 基金项目： | 国家自然科学基金项目(42077150；31672228)。 |
| |
| 摘 要： | 【目的】 比较加硼和不加硼条件下豌豆根尖细胞壁组分对铝的吸附解吸特征的差异，探讨硼对植物铝胁迫的缓解机制。 【方法】 以中豌6号豌豆(Pisum Sativum)为试验材料，在硼(0.6 μmol/L)水平下水培6天，提取豌豆根尖1 cm段细胞壁各组分并进行铝吸附解吸试验，根尖细胞壁各组分分别为螯合态果胶(果胶1)，碱溶态果胶(果胶2)，半纤维素和纤维素，并分析3个不同硼处理 (无硼、50 μmol/L H3BO3和 50 μmol/L 3-硝基苯硼酸) 条件下各组分对铝吸附解吸的影响。 【结果】 豌豆根尖细胞壁各组分含量为:纤维素>半纤维素>果胶2>果胶1。硼能够与果胶1和果胶2发生络合反应，与半纤维素也可能发生络合反应，从而影响果胶1、果胶2和半纤维素对铝的吸附解吸。在铝胁迫下，根尖细胞壁中的果胶是主要的铝结合位点，以果胶2结合最多。与对照比，硼处理显著提高了果胶2对铝的吸附量，但解吸量变化不显著。pH 3.5条件下，硼酸与3-硝基苯硼酸处理相比，更能有效地影响果胶对铝的吸附解吸。因此将铝固定在细胞壁的果胶2内，可能是硼酸缓解铝毒的重要机制之一。 【结论】 细胞壁是铝的主要结合部位，细胞壁果胶2是根尖细胞壁主要的铝结合位点，硼与果胶2的结合可能是硼缓解铝毒的重要机制之一。
|
| 关 键 词： | 豌豆 硼酸 3-硝基苯硼酸 果胶 铝胁迫 吸附解吸 |
| 收稿时间： | 2022-01-29 |
Effects of boron on aluminum adsorption and desorption of cell wall components of pea root tips |
| |
| FENG Ying-ming,LUO Gong-rong,QU Mei,XUAN Zu-ying,LI Xue-wen,Mai Jing-wen,YU Min.Effects of boron on aluminum adsorption and desorption of cell wall components of pea root tips[J].Plant Nutrition and Fertilizer Science,2022,28(10):1893-1900. |
| |
| Authors: | FENG Ying-ming LUO Gong-rong QU Mei XUAN Zu-ying LI Xue-wen Mai Jing-wen YU Min |
| |
| Affiliation: | 1.International Research Centre for Environmental Membrane Biology / Department of Horticulture School of Food Science and Engineering, Foshan University, Foshan, Guangdong 528200 |
| |
| Abstract: | 【Objectives】 Comparing the difference of aluminum adsorption and desorption characteristics of cell wall components pea root tips under boron and non-boron conditions to reveal the alleviating mechanism of boron on aluminum stress in plants. 【Methods】 Seeds of pea (Pisum sativum) were hydroponically cultured with Hoagland solution at 0.6 μmol/L B (H3BO3) level for 6 days, then the root tips were collected and the different cell wall components were extracted, including chelated pectin (pectin 1), alkaline-soluble pectin (pectin 2), hemicellulose, and cellulose. They were used to study the adsorption and desorption of aluminum under different boron treatments (i.e. no B, 50 μmol/L H3BO3, 50 μmol/L 3-NBA). 【Results】 The content of each component in the cell wall of pea root tips was cellulose>hemicellulose>pectin 2>pectin 1. The results revealed that boron may combine with pectin 1, pectin 2, and hemicellulose, thereby affecting their adsorption and desorption capacity for aluminum. Under aluminum toxicity, the pectin in the root cell wall was the major aluminum binding site, especially pectin 2. Further, pectin 2 came from the cell wall with a boron supply adsorbed much more aluminum, but the desorption amount didn’t change significantly. At pH 3.5, compared with 3-nitrophenylboronic acid (3-NBA), boron more effectively affected the adsorption and desorption of aluminum by pectin. Therefore, the immobilization of aluminum in pectin 2 may be one of the important mechanisms for boric acid to alleviate aluminum toxicity. 【Conclusions】 Pectin 2 is the main aluminum binding site in root tip cell wall, and the binding of boron to pectin 2 is an important mechanism of boron alleviating aluminum toxicity. |
| |
| Keywords: | |
|
| 点击此处可从《植物营养与肥料学报》浏览原始摘要信息 |
|
点击此处可从《植物营养与肥料学报》下载全文 |
|
