| 镉安全水稻材料根系细胞壁果胶对镉的响应特征 |
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| 引用本文： | 杨釜,余海英,黄化刚,李芹,李廷轩.镉安全水稻材料根系细胞壁果胶对镉的响应特征[J].植物营养与肥料学报,2023,29(4):704-711. |
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| 作者姓名： | 杨釜 余海英 黄化刚 李芹 李廷轩 |
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| 作者单位： | 1.四川农业大学资源学院，四川成都 611130 |
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| 基金项目： | 四川省区域创新合作项目(22QYCX0104)；德阳市“揭榜挂帅”科技计划项目(2021JBJZ016)。 |
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| 摘 要： | 【目的】研究镉(Cd)处理下水稻根系细胞壁果胶对Cd胁迫的响应，进一步深化Cd安全水稻材料根系细胞壁Cd的固持机制。【方法】以Cd安全水稻材料D62B为研究对象，普通材料Luhui17为对照进行水培试验。设4个Cd质量浓度处理：0 mg/L (CK)、0.5 mg/L (Cd0.5)、1.0 mg/L (Cd1)、2.0 mg/L (Cd2)。在水稻分蘖期采集根系样品，分析细胞壁多糖中果胶、半纤维1、半纤维2以及残渣部分的Cd含量，测定果胶糖醛酸含量、果胶酯化度、果胶甲酯酶(PME)活性、根系过氧化氢(H2O2)含量以及细胞壁过氧化物酶(POD)活性，进而分析根系细胞壁果胶对Cd的响应特征。【结果】1) Cd胁迫下，D62B和Luhui17根系细胞壁果胶合成增加，根系细胞壁低酯化和高酯化果胶糖醛酸含量均表现为D62B高于Luhui17。Cd处理下D62B根系细胞壁低酯化和高酯化果胶糖醛酸含量较对照分别增加了13.21%～71.82%和22.10%～64.27%,Luhui17分别增加了24.14%～137.86%和13.12%～41.26%。...
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| 关 键 词： | 镉安全水稻材料 细胞壁 果胶 去甲酯化作用 |
| 收稿时间： | 2022-08-24 |
The characteristics of cell wall pectin in roots of cadmium-safe rice line in response to cadmium stress |
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| YANG Fu,YU Hai-ying,HUANG Hua-gang,LI Qin,LI Ting-xuan.The characteristics of cell wall pectin in roots of cadmium-safe rice line in response to cadmium stress[J].Plant Nutrition and Fertilizer Science,2023,29(4):704-711. |
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| Authors: | YANG Fu YU Hai-ying HUANG Hua-gang LI Qin LI Ting-xuan |
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| Affiliation: | 1.College of Resource, Sichuan Agricultural University, Chengdu, Sichuan 611130, China |
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| Abstract: | 【Objectives】 We compared the properties of cell wall pectin in roots of cadmium (Cd)-safe and common rice line when exposure to Cd stress, to fully understand the mechanism of Cd retention by root of Cd-safe rice line. 【Methods】 A hydroponic experiment was conducted using a Cd-safe rice line D62B and a common rice line Luhui17 as the comparing material. The Cd concentration of nutrient solution included 0 (CK), 0.5 mg/L (Cd0.5), 1.0 mg/L (Cd1) and 2.0 mg/L (Cd2). At the tillering stage, root samples were collected. The Cd concentrations in cell wall polysaccharides, including pectin, hemicellulose 1, hemicellulose 2 and residue fraction, were determined respectively. Furthermore, the uronic acid concentration of pectin, pectin esterification degree, pectin methyl esterase activity (PME), root hydrogen peroxide (H2O2) concentration and cell wall peroxidase (POD) activity were detected, so as to clarify the response characteristics of root cell wall pectin to Cd. 【Results】 1) Cd treatment promoted the pectin synthesis of root cell wall in both rice lines, and the uronic acid concentrations of low and high esterified pectin in the root cell wall of D62B were higher than those of Luhui17. Compared with CK, the uronic acid concentrations of low esterified and high esterified pectin in the root cell wall of D62B were increased by 13.21%–71.82%, 22.10%–64.27%, and those in Luhui17 were increased by 24.14%–137.86%, 13.12%–41.26%, respectively, under Cd treatment. 2) The pectin esterification degree and PME activity in root cell wall of D62B and Luhui17 were higher than those of CK under Cd1 and Cd2 treatment. The PME activity in root cell wall of D62B was significantly higher than that of Luhui17 under the same Cd stress. 3) Cd induced H2O2 accumulation and influenced peroxidase activity in the roots of both rice lines. Under Cd stress, the H2O2 concentration and peroxidase activity of D62B roots were lower than those of Luhui17, indicating that pectin methyl esterase had stronger demethylation effect on pectin. 【Conclusions】 When exposed to Cd stress, Cd-safe rice line D62B showed stronger ability of low and high esterified pectin synthesis and had higher PME activity in its root cell wall than that of common material, leading to provide more Cd binding sites and show stronger Cd binding ability, which was conducive to the retention of Cd in D62B roots. |
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