| 火龙果总黄酮提取工艺优化 |
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| 引用本文： | 孙艳,杨志伟.火龙果总黄酮提取工艺优化[J].南方农业学报,2016,47(6):1001-1008. |
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| 作者姓名： | 孙艳 杨志伟 |
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| 作者单位： | 广西大学 轻工与食品工程学院,南宁,530004广西大学 轻工与食品工程学院，南宁 530004广西高校特色农产品精深加工与安全控制重点实验室，南宁 530004 |
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| 基金项目： | 广西高校特色农产品精深加工与安全控制重点实验室项目(2014) |
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| 摘 要： | 【目的】优化火龙果总黄酮提取工艺条件,为火龙果的综合开发与利用提供参考依据。【方法】以红皮红肉种火龙果为试验材料,在单因素试验的基础上,采用正交试验设计优化火龙果总黄酮的乙醇回流提取工艺,并通过响应面法优化其超声波辅助提取工艺,确定最佳提取工艺条件。【结果】乙醇回流提取火龙果总黄酮的最佳工艺条件为:乙醇体积分数80%、提取温度80℃、料液比1∶25、提取时间2 h,在此条件下火龙果总黄酮提取量为18.07 mg/g;超声波辅助提取火龙果总黄酮的最佳工艺条件为:超声波功率250 W、乙醇体积分数80%、料液比1∶25、提取温度80℃、超声波时间20 min,在此条件下火龙果总黄酮提取量为19.58 mg/g。【结论】超声波辅助提取法操作简单、耗时短、提取效率高,效果优于乙醇回流提取法,可用于火龙果总黄酮的工业化提取。
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| 关 键 词： | 火龙果 总黄酮 提取工艺 正交试验设计 响应面法 |
Optimization for extraction process of total flavonoids from pitaya |
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| SUN Yan,YANG Zhi-wei.Optimization for extraction process of total flavonoids from pitaya[J].Journal of Southern Agriculture,2016,47(6):1001-1008. |
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| Authors: | SUN Yan YANG Zhi-wei |
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| Abstract: | Objective]The extraction conditions of total flavonoids from pitaya was optimized , in order to provide a theoretical basis for comprehensive development and utilization of pitaya. Method]Using red-peel red-pulp pitaya as ma-terial, based on single-factor experiment, the ethanol refluxing extraction process of total flavonoids from pitaya was opti-mized using orthogonal experiment design. Meanwhile , the ultrasonic-assisted extraction process was optimized using re-sponse surface methodology. Result]The results showed that, the optimal conditions of ethanol refluxing extraction were 80%ethanol, 80℃of extraction temperature, 1∶25 of solid-to-liquid ratio, and 2 h of extraction time. Under those opti-mal conditions, the yield of total flavonoids from pitaya was 18.07 mg/g. The optimal conditions of ultrasonic-assisted ex-traction were as follows:250 W of ultrasonic power, 20 min of ultrasonic time, 1∶25 of solid-to-liquid ratio, 80%ethanol, and 80℃of extraction temperature. Under those optimal conditions, the yield of total flavonoids was 19.58 mg/g. Con-clusion]The ultrasonic-assisited extraction is simple, time-saving, high-efficiency, and better than ethanol refluxing ex-traction. Therefore, the ultrasonic-assisited extraction can be used to extract total flavonoids from pitata. |
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| Keywords: | pitaya total flavonoids extraction process orthogonal experiment design response surface methodology |
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