| 响应面法优化近江牡蛎多糖提取工艺 |
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| 引用本文： | 牛改改,游刚,张晨晓,余振宁. 响应面法优化近江牡蛎多糖提取工艺[J]. 南方农业学报, 2016, 47(6): 994-1000. DOI: 10.3969/j:issn.2095-1191.2016.06.994 |
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| 作者姓名： | 牛改改 游刚 张晨晓 余振宁 |
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| 作者单位： | 钦州学院 食品工程学院，广西 钦州 535000广西北部湾海洋生物多样性养护重点实验室/北部湾海洋生物资源开发与保护重点实验室，广西钦州 535000广西北部湾特色海产品资源开发与高值化利用高校重点实验室，广西 钦州 535000钦州学院 食品工程学院,广西 钦州,535000 |
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| 基金项目： | 广西北部湾海洋生物多样性养护重点实验室项目(2015ZC09)；北部湾海洋生物资源开发与保护重点实验室项目(2015ZC15) |
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| 摘 要： | 【目的】优化近江牡蛎多糖提取工艺,为不同海域牡蛎多糖含量对比及其结构分析打下基础。【方法】以近江牡蛎为原料,通过单因素试验考察液料比、提取温度、提取时间、提取次数4个因素对其多糖提取率的影响,并在此基础上选取液料比、提取时间及提取温度3个因素为变量,近江牡蛎多糖提取率为考察指标,采用Box-Behnken试验设计方法建立回归模型,以优化近江牡蛎多糖提取工艺条件。【结果】通过响应面法建立近江牡蛎多糖提取率(Y)与液料比(A)、提取时间(B)、提取温度(C)的二次多项式数学模型为:Y=9.65-0.18A-0.071B+0.080C-0.27AB-0.24AC-0.52BC-2.6A2-1.34B2-1.35C2,该模型拟合度好;最佳提取工艺条件为:液料比90∶1、提取温度90℃、提取时间3 h、提取次数1次,在此条件下的近江牡蛎多糖提取率为9.72%,与预测值(9.66%)相对偏差为0.62%。【结论】采用响应面分析法优化近江牡蛎多糖提取工艺具有可行性,可用于实际生产,以提高多糖提取率。
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| 关 键 词： | 近江牡蛎 多糖 响应面分析 提取工艺优化 |
Optimization for extraction process of polysaccharide from Ostrea rivularis Gould by response surface methodology |
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| NIU Gai-gai,YOU Gang,ZHANG Chen-xiao,YU Zhen-ning. Optimization for extraction process of polysaccharide from Ostrea rivularis Gould by response surface methodology[J]. Journal of Southern Agriculture, 2016, 47(6): 994-1000. DOI: 10.3969/j:issn.2095-1191.2016.06.994 |
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| Authors: | NIU Gai-gai YOU Gang ZHANG Chen-xiao YU Zhen-ning |
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| Abstract: | Objective]The present experiment was conducted to optimize the extraction process of polysaccharide from Ostrea rivularis Gould, in order to provide a theoretical basis for comparing and analyzing content and structure of oyster polysaccharide in different sea areas. [Method]The effects of liquid-solid ratio, extraction temperature, extraction time and extraction times on extraction rate of polysaccharides from O. rivularis were investigated by single-factor test. Mean-while, based on the results of single-factor test, liquid-solid ratio, extraction time and extraction temperature was chosen as independent variables, and the extraction rate of oyster polysaccharide was selected as response value. Then the regres-sion model was established by Box-Behnken experiment design method, so as to optimize extraction process of polysaccha-ride from O. rivularis. [Result]The mathematical model for quadratic polynomial of polysaccharide extraction rate (Y) with liquid-solid ratio(A), extraction time(B), extraction temperature(C) was established by response surface method, as fol-low:Y=9.65-0.18A-0.071B+0.080C-0.27AB-0.24AC-0.52BC-2.6A2-1.34B2-1.35C2, with high fitting degree. The opti-mum conditions of extraction process were as follows:90∶1 of liquid-solid ratio, 90℃of extraction temperature, 3 h of ex-traction time, extracting 1 time. Under the above optimum conditions, the extraction rate of polysaccharide from O. rivularis reached up to 9.72%, which was close to the predicted value(9.66%), with relative deviation of 0.62%. [Conclusion]The response surface methodology is a workable method to optimize extraction conditions of polysaccharide from O. rivularis, which should be applied to increase effectively extraction rate of polysaccharides in actual production. |
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| Keywords: | Ostrea rivularis Gould polysaccharide response surface analysis optimization for extraction process |
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