| CO_2脱涩对柿细胞壁果胶构成、提取及理化性质的影响 |
| |
| 引用本文： | 张雪丹,辛力,杨娟侠,卢昊,孙山,艾呈祥.CO_2脱涩对柿细胞壁果胶构成、提取及理化性质的影响[J].南方农业学报,2017,48(3). |
| |
| 作者姓名： | 张雪丹 辛力 杨娟侠 卢昊 孙山 艾呈祥 |
| |
| 作者单位： | 山东省果树研究所,山东 泰安,271000 |
| |
| 基金项目： | 山东省自然科学基金项目，山东省现代农业产业技术体系水果创新团队专项基金项目 |
| |
| 摘 要： | 【目的】比较不同程度CO_2脱涩处理的涩柿细胞壁果胶组成差异,分析脱涩对柿果果胶提取率和理化性质的影响,为涩柿果胶的高效提取和应用提供技术支持。【方法】以金瓶柿为试材,分别进行未脱涩处理及采用90%~95%CO_2处理15 h后室温放置24 h、处理24 h后室温放置24 h、处理40 h后室温放置48 h 3种脱涩处理。通过福林酚法测定柿果可溶性和不溶性单宁含量,半乳糖醛酸法测定细胞壁果胶组分含量;利用超声波辅助酸提法提取柿果果胶,并分析果胶提取率、色泽、半乳糖醛酸含量、酯化度、单糖组成等理化性质。【结果】随着CO_2脱涩处理时间的延长,柿果可溶性单宁含量降低,不溶性单宁含量升高,水溶性果胶含量先降低后升高,螯合性果胶和酸溶性果胶含量先升高后降低;果胶提取率随柿果脱涩程度的提高而升高,90%~95%CO_2处理40 h后室温放置48 h的柿果果胶提取率是未脱涩柿果的4.00倍;提取的果胶亮度L*显著降低(P0.05,下同),半乳糖醛酸含量显著升高;提取的果胶均为高甲氧基果胶,均含有鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖和半乳糖醛酸,其中阿拉伯糖和木糖相对百分含量随着脱涩时间的延长而逐渐降低,半乳糖醛酸相对百分含量则逐渐升高。【结论】CO_2脱涩处理可显著提高柿果果胶提取率,并影响提取果胶的理化性质,因此脱涩可作为制备柿果果胶的前处理工序,以90%~95%CO_2处理柿果40 h后室温放置48 h的脱涩方式提取得到的果胶品质更佳。
|
| 关 键 词： | 柿 CO2脱涩 果胶组成 理化性质 可溶性单宁 |
Effects of deastringency treatments with CO2 on composition,yield and physicochemical properties of pectin extracted from persimmon cell wall |
| |
| ZHANG Xue-dan,XIN Li,YANG Juan-xia,LU Hao,SUN Shan,AI Cheng-xiang.Effects of deastringency treatments with CO2 on composition,yield and physicochemical properties of pectin extracted from persimmon cell wall[J].Journal of Southern Agriculture,2017,48(3). |
| |
| Authors: | ZHANG Xue-dan XIN Li YANG Juan-xia LU Hao SUN Shan AI Cheng-xiang |
| |
| Abstract: | Objective]The present study compared difference of pectin composition in persimmon cell wall at different deastringency degrees by CO2, analyzed the effects of deastringency treatment on extraction yield and physicochemical properties of persimmon pectin, in order to provide technical support for efficient extraction and application of persimmon pectin. Method]Jinping persimmon was subjected to deastringency treatment through four experiments: no deastringency treatment, deastringency treatment with 90%-95% CO2 for 15,24,40 h and placed at ambient temperature for 24,24,48 h. Folin-ciocaileu method was used to detect content of soluble tannin and condensed tannin. Polygalacturonic acid method was chosen to detect the pectin composition in cell wall. Pectin was extracted by ultrasound-assisted acid method. Physico-chemical properties of the extracted pectin were investigated, including pectin yield, color, polygalacturonic acid content, esterification degree and monosaccharides compositions. Result]As deastringency time extended, content of soluble tannin decreased, content of condensed tannin increased, water soluble pectin decreased at first and then increased while chelator soluble pectin and acid soluble pectin showed the opposite tendency in the process of deastringency. With increase of deas-tringency degree, extraction yield of pectin increased,pectin yield of persimmon with 90%-95% CO2 treatment 40 h fol-lowed by being placed at ambient temperature for 48 h was 4.00 times as that of untreated persimmon. The lightness L* of extracted pectin decreased significantly(P<0.05,the same below), polygalacturonic acid content increased significantly. The pectin extracted was high methoxyl pectin, contained rhamnose, arabinose, galactose, glucose, xylose and galacturonic acid. As deastringency time prolonged, relative percentage of arabinose and xylose decreased, but that of galacturonic acid increased. Conclusion]Deastringency treatment with CO2 can significantly increase extraction yield of pectin , affect physicochemical properties of pectin, hence it can be taken as pre-treatment in production of persimmon pectin. Deastrin-gency treatment with 90%-95% CO2 for 40 h and put in ambient temperature for 48 h can produce the pectin with optimal quality. |
| |
| Keywords: | persimmon deastringency treatment with CO2 pectin composition physicochemical property soluble tannin |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
