| 脂肪来源间充质干细胞条件培养基对小型猪腹腔镜肝损伤氧化应激反应的影响 |
| |
| 引用本文： | 焦智慧,张千振,王月,刘涛,刘博洋,马亚军,刘笑凝,朴晨曦,王洪斌. 脂肪来源间充质干细胞条件培养基对小型猪腹腔镜肝损伤氧化应激反应的影响[J]. 畜牧兽医学报, 2021, 52(6): 1734-1743. DOI: 10.11843/j.issn.0366-6964.2021.06.028 |
| |
| 作者姓名： | 焦智慧 张千振 王月 刘涛 刘博洋 马亚军 刘笑凝 朴晨曦 王洪斌 |
| |
| 作者单位： | 1. 东北农业大学动物医学学院, 哈尔滨 150030;2. 东北林业大学野生动物与自然保护地学院, 哈尔滨 150040 |
| |
| 基金项目： | 国家自然科学基金项目（31972757；31772807） |
| |
| 摘 要： | 旨在探究脂肪来源间充质干细胞条件培养基(adipose-derived mesenchymal stem cells-conditioned medi-um,ADSCs-CM)对小型猪肝损伤氧化应激反应的影响,作者选取24头健康小型猪,随机分为4组,每组6只,分别为模型组(IRI)、DMEM对照组(DMEM)、ADSC...
|
| 关 键 词： | 小型猪 腹腔镜 肝缺血再灌注合并部分肝切除损伤 脂肪间充质干细胞条件培养基 氧化应激 |
| 收稿时间： | 2020-11-12 |
Effect of Adipose-derived Stem Cells-conditioned Medium on Oxidative Stress in Miniature Pigs with Laparoscopic Hepatic Injury |
| |
| JIAO Zhihui,ZHANG Qianzhen,WANG Yue,LIU Tao,LIU Boyang,MA Yajun,LIU Xiaoning,PIAO Chenxi,WANG Hongbin. Effect of Adipose-derived Stem Cells-conditioned Medium on Oxidative Stress in Miniature Pigs with Laparoscopic Hepatic Injury[J]. Chinese Journal of Animal and Veterinary Sciences, 2021, 52(6): 1734-1743. DOI: 10.11843/j.issn.0366-6964.2021.06.028 |
| |
| Authors: | JIAO Zhihui ZHANG Qianzhen WANG Yue LIU Tao LIU Boyang MA Yajun LIU Xiaoning PIAO Chenxi WANG Hongbin |
| |
| Affiliation: | 1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;2. College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China |
| |
| Abstract: | To explore the oxidative stress response of adipose-derived mesenchymal stem cells-conditioned medium (ADSCs-CM) on liver injury in miniature pigs. In this experiment, 24 healthy miniature pigs were selected and randomly divided into four groups, namely model group (IRI), DMEM control group (DMEM), ADSCs-CM treatment group (CM) and ADSCs treatment Groups (ADSCs), six pigs each group. All four groups, laparoscopic techniques were used to establish the liver injury model of miniature pigs with ischemia reperfusion (IR) combined with partial hepatectomy. The pigs in the IRI group were transplanted with normal saline, the pigs in the DMEM group were transplanted with concentrated basal medium, and the pigs in the CM group were transplanted with concentrated adipose-derived stem cell culture medium, the pigs in the ADSCs group were transplanted with adipose stem cells. Blood and liver tissue samples were collected in each group before the operation, 1 day, 3 days and 7 days after the operation. Use liver function test kit to detect total bilirubin (T-BIL), lactate dehydrogenase (LDH), and total protein (TP) in sera; use oxidation reaction kit to detect malondialdehyde (MDA), intramedullary peroxidase (MPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase in liver tissue (GSH-Px) for detection. Results were as follows:1 day and 3 days after operation, the model group and the control group had severe liver function damage, with obvious oxidative stress. The CM and ADSCs treatment groups significantly promoted the recovery of liver function, and the expression of oxidative stress related indicators decreased significantly compared with the model group and the control group. After 7 days, each group basically recovered to the preoperative level. The results showed that laparoscopic liver ischemia-reperfusion combined with partial hepatectomy injury can cause oxidative stress in miniature pigs. Adipose-derived mesenchymal stem cells and their condition medium can improve oxidative stress after liver injury. |
| |
| Keywords: | miniature pigs laparoscopy liver ischemia-reperfusion combined with partial hepatectomy injury adipose stem cells-conditioned medium oxidative stress |
|
| 点击此处可从《畜牧兽医学报》浏览原始摘要信息 |
|
点击此处可从《畜牧兽医学报》下载全文 |
|
