Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis |
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| Authors: | L von Gersdorff Jørgensen P W Kania K J Rasmussen A H Mattsson J Schmidt A Al‐Jubury A Sander A Salanti K Buchmann |
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| Affiliation: | 1. Laboratory of Aquatic Pathobiology at the Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark;2. Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark;3. Evaxion Biotech, Copenhagen, Denmark;4. Centre for Medical Parasitology at the Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark |
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| Abstract: | The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n‐terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I‐antigen in future vaccine trials. |
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| Keywords: | parasite Ichthyophthirius multifiliis rainbow trout subunit vaccine vaccine |
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