| 淡紫紫孢菌微菌核发酵滤液生防活性及代谢组学分析 |
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| 引用本文： | 范乐乐,郭妍,赵雪,孙漫红,李世东.淡紫紫孢菌微菌核发酵滤液生防活性及代谢组学分析[J].中国生物防治学报,2022,38(4):821-830. |
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| 作者姓名： | 范乐乐 郭妍 赵雪 孙漫红 李世东 |
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| 作者单位： | 中国农业科学院植物保护研究所, 北京 100193 |
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| 基金项目： | 国家重点研发计划(2019YFD1002000);现代农业产业技术体系项目(CARS-23-C04);广西重大研发计划(桂科AB21220030) |
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| 摘 要： | 淡紫紫孢菌是一种重要的生防真菌,在特定培养条件下可产生抗逆结构-微菌核。本文通过室内生测和温室盆栽试验,研究了淡紫紫孢菌微菌核发酵滤液对植物病原菌和根结线虫的抑制作用。结果表明,微菌核发酵滤液具有较强的杀线抑菌活性,其中对核盘菌生长抑制率达到70.5%,并显著降低菌核数量,滤液稀释10倍后对根结线虫二龄幼虫的致死率达到50%以上。用量为10 mL/株时对根结线虫病防效达到44.9%,黄瓜株高和茎粗分别增加21.6%和38.4%。同时,基于LC-MS构建了淡紫紫孢菌微菌核培养代谢组。分析表明,微菌核形成过程中芥酸、胡椒酚和泛酸等抑菌物质以及丁酸、咪唑乙酸等杀线化合物相对含量显著增加。本研究为揭示淡紫紫孢菌生防机制奠定了基础,为微菌核制剂的研发提供了理论依据。
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| 关 键 词： | 淡紫紫孢菌 微菌核 根结线虫 发酵滤液 代谢组 |
| 收稿时间： | 2022-06-26 |
Biocontrol Activities and Metabolome Analysis of Microsclerotia Fermentation Filtrate of Purpureocillium lilacinum |
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| FAN Lele,GUO Yan,ZHAO Xue,SUN Manhong,LI Shidong.Biocontrol Activities and Metabolome Analysis of Microsclerotia Fermentation Filtrate of Purpureocillium lilacinum[J].Chinese Journal of Biological Control,2022,38(4):821-830. |
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| Authors: | FAN Lele GUO Yan ZHAO Xue SUN Manhong LI Shidong |
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| Affiliation: | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | Purpureocillium lilacinum is an important biocontrol fungus,which can produce a kind of resistant structure -microsclerotia under given culture conditions.In this study,the control efficiencies of microsclerotia fermentation filtrate of P.lilacinum against plant fungal pathogens and root-knot nematode were investigated by antagonism in the lab and pot experiment in greenhouse.The results showed that the filtrate of microsclerotia had strong inhibition to nematodes and pathogenic fungi.The growth inhibition rate to Sclerotinia sclerotiorum reached 70.5%,and the yield of sclerotia significantly decreased.The nematicidal activity of the filtrate to the juveniles of root-knot nematode was over 50% when the filtrate was diluted 10 times.In greenhouse,when 10 mL/seedling was used,the control efficiency against root-knot nematode in cucumber reached 44.9%,and the height and stem diameter of the seedlings increased by 21.6% and 38.4%,respectively.Additionally,the metabolome of P.lilacinum microsclerotia fermentation was constructed basing on LC-MS analysis,and the results showed that the relative contents of anti-microbial compounds,such as erucic acid,piperol and pantothenic acid,and the metabolites with nematicidal activities such as butyric acid and imidazolacetic acid,increased significantly.This study lays a foundation to understand the biocontrol mechanisms of P.lilacinum and facilitates the development of fungal agents composed of microsclerotia. |
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| Keywords: | Purpureocillium lilacinum microsclerotia root-not nematode egg fermentation filtrate metabolome |
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