| 荷斯坦奶牛不同群体牛舍土壤细菌菌群结构差异分析 |
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| 引用本文： | 杨思瑞,杨卓,火苗,张洁,张力莉,李胜利,徐晓锋.荷斯坦奶牛不同群体牛舍土壤细菌菌群结构差异分析[J].浙江农业学报,2022,34(2):275. |
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| 作者姓名： | 杨思瑞 杨卓 火苗 张洁 张力莉 李胜利 徐晓锋 |
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| 作者单位： | 1.宁夏大学 农学院,宁夏 银川 7500212.中国农业大学 动物科学技术学院,动物营养学国家重点实验室,北京 100193 |
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| 基金项目： | 宁夏重点研发中西部合作项目(20190410110) |
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| 摘 要： | 为探究荷斯坦奶牛不同群体牛舍土壤细菌菌群结构差异,本试验于宁夏某规模化荷斯坦奶牛场不同群体牛舍分别采集土壤样品,利用16S rDNA测序测定其细菌菌群结构。结果表明:不同群体牛舍土壤细菌菌群的OTU数目与Alpha多样性均无显著差异(P>0.05),主坐标分析(PCoA)结果显示,不同群体牛舍土壤细菌菌群的Beta多样性差异明显,高产泌乳牛舍土壤与低产泌乳牛舍土壤的细菌菌群结构较接近。不同群体奶牛牛舍土壤的优势菌门 (丰度>10%)基本为变形菌门、拟杆菌门、放线菌门与厚壁菌门,泌乳牛舍土壤中放线菌门的丰度显著高于其他群体牛舍土壤(P<0.05),哺乳犊牛岛土壤中软壁菌门与Gemmatimonadates的丰度极显著高于其他群体牛舍土壤(P<0.01)。不同群体奶牛牛舍土壤中优势菌属(丰度>3%)差异明显,各牛舍土壤的优势菌属中,Proteiniphilum、Fermentimonas、Truepera等蛋白质降解菌在非泌乳阶段牛舍土壤中的丰度较高;脱硫杆菌属、盐单胞菌属、硫碱螺旋菌属等嗜盐碱的菌属在干奶牛舍土壤与断奶犊牛舍土壤中的丰度较高;不动杆菌属与鞘脂杆菌属等致病菌在高产泌乳牛舍土壤中的丰度较高。
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| 关 键 词： | 荷斯坦奶牛 不同群体 牛舍土壤 细菌 |
| 收稿时间： | 2020-09-18 |
Analysis on difference of soil bacterial community structure in different groups of Holstein dairy cow barns |
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| YANG Sirui,YANG Zhuo,HUO Miao,ZHANG Jie,ZHANG Lili,LI Shengli,XU Xiaofeng.Analysis on difference of soil bacterial community structure in different groups of Holstein dairy cow barns[J].Acta Agriculturae Zhejiangensis,2022,34(2):275. |
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| Authors: | YANG Sirui YANG Zhuo HUO Miao ZHANG Jie ZHANG Lili LI Shengli XU Xiaofeng |
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| Affiliation: | 1. School of Agriculture, Ningxia University, Yinchuan 750021, China
2. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China |
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| Abstract: | In order to understand the differences of soil bacterial flora structure in different groups of Holstein dairy cows, soil samples were collected from different groups of cow barns in a large-scale Holstein dairy farm in Ningxia, and the bacterial flora structure was determined by 16S rDNA sequencing. The results showed that there was no significant difference between OTU number and Alpha diversity of soil bacterial flora in different groups of cow barns (P>0.05). The results of principal coordinate analysis (PCoA) showed that there were obviously differences in Beta diversity of soil bacterial flora in different groups of cow barns, and the structure of bacterial flora in the soil of high-yielding lactating cow barns was similar to that of low-yielding lactating cow barns. The dominant bacteria (relative abundance>10%) at phylum level in different groups of cow barn soil were Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The abundance of Actinobacteria in lactating cow barn soil was significantly higher than that in other groups (P<0.05). The abundance of Tenericutes and Gemmatimonadates in pre-weaned calf hutch soil was significantly higher than that in other groups (P<0. 01). The dominant bacteria (relative abundance>3%) in the soil of different groups of cow barns were obviously different at the genus level. Among these dominant bacteria, protein-degrading bacteria such as Proteiniphilum, Fermentimonas and Truepera had more abundance in the soil of cow barns at the non-lactation stage; the saline-alkali tolerant bacteria such as Desulfobacterium, Halomonas and Thioalkalispira had more abundance in the soil of dry cow barns and post-weaned calf barns. Pathogenic bacteria such as Acinetobacter and Sphingobacterium had more abundance in the soil of high-yielding lactating cow barns. |
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| Keywords: | Holstein cows different groups barn soil bacteria |
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