| 猕猴桃灰霉病拮抗解淀粉芽孢杆菌X17可湿性粉剂的研制 |
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| 引用本文： | 钱一鑫,康冀川,罗乙凯,耿坤,雷帮星,张斌.猕猴桃灰霉病拮抗解淀粉芽孢杆菌X17可湿性粉剂的研制[J].中国生物防治学报,2016,32(3):342-348. |
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| 作者姓名： | 钱一鑫 康冀川 罗乙凯 耿坤 雷帮星 张斌 |
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| 作者单位： | 1. 贵州大学西南药用生物资源教育部工程研究中心, 贵阳 550025;
2. 贵州大学药学院, 贵阳 550025;
3. 贵州大学烟草学院, 贵阳 550025;
4. 贵阳市植保质检站, 贵阳 550081 |
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| 基金项目： | 贵州省农业科技攻关计划项目(黔科合NY(2013)3044号;黔科合NY(2013)3042号)，贵州大学引进人才科研项目(贵大人基合字(2014)59号)，国家自然科学基金(31460011)，贵州省省校合作计划项目(黔科合LH字(2014)7657号) |
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| 摘 要： | 以植物内生解淀粉芽孢杆菌X17为研究对象,通过对载体、分散剂、润湿剂、稳定剂、保护剂的筛选,确定了内生解淀粉芽孢杆菌X17可湿性粉剂最佳配方:硅藻土10%,羧甲基纤维素钠4%,十二烷基硫酸钠6%,甲基纤维素2%,糊精0.1%。结果显示,制备的解淀粉芽孢杆菌X17可湿性粉剂在25℃室温下芽孢含量为2.0×1010cfu/g,pH7.0,悬浮率78%,润湿时间35s,细度通过率99%,制剂贮藏6个月存活率达78%。盆栽试验结果表明,内生解淀粉芽孢杆菌X17可湿性粉剂和母粉500倍液对猕猴桃灰霉病的保护效果分别为80.59%和68.82%,菌株X17可湿性粉剂的防治效果优于其母粉。
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| 关 键 词： | 解淀粉芽孢杆菌 猕猴桃灰霉病菌 可湿性粉剂 生防效果 |
| 收稿时间： | 2015-07-17 |
Development of Wettable Powder of Bacillus amyloliquefaciens X17 for Control of Botrytis cinerea on Kiwifruits |
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| QIAN Yixin,KANG Jichuan,LUO Yikai,GENG Kun,LEI Bangxing,ZHANG Bin.Development of Wettable Powder of Bacillus amyloliquefaciens X17 for Control of Botrytis cinerea on Kiwifruits[J].Chinese Journal of Biological Control,2016,32(3):342-348. |
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| Authors: | QIAN Yixin KANG Jichuan LUO Yikai GENG Kun LEI Bangxing ZHANG Bin |
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| Affiliation: | 1. The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang 550025, China;
2. College of Pharmay, Guizhou University, Guiyang 550025, China;
3. College of Tobacco Science, Guizhou University, Guiyang 550025, China;
4. Guiyang Plant Protection and Quarantine Station, Guiyang 550081, China |
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| Abstract: | Bacillus amyloliquefaciens X17, a plant endophyte, was used as raw material to develop its wettable powder. After carefully screening the influential factors including carrier, dispersing agent, wetting agent, stabilizer, and protective agent, the optimal formulation was determined to be 10% diatomite as filler, 4% CMC-Na as dispersing agent, 6% SDS as wetting agent, 2% methylcellulose as stabilizer, 0.1% dextrin as UV protective agent. The spore content of the wettable powder with a suspension rate of 78% and a dispersing time of 35 s, reached 2.0×1010 cfu/g (pH 7.0) in the quality evaluation investigation. Pot experiment showed that the protective effects of the diluted 500 times solutions of the prepared wettable powder and initial fermented powder were 80.59% and 68.82%, respectively. Importantly, its control efficacy against Botrytis gray mold of kiwifruit was superior to that of the initial fermented powder. |
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| Keywords: | Bacillus amyloliquefaciens Botrytis cinerea wettable powder biocontrol effect |
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