| 香蕉枯萎病生防菌肥的优化及其防病促生效果研究 |
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| 引用本文： | 杜婵娟,杨迪,潘连富,叶云峰,张晋,付岗.香蕉枯萎病生防菌肥的优化及其防病促生效果研究[J].中国生物防治学报,2020,36(3):396-404. |
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| 作者姓名： | 杜婵娟 杨迪 潘连富 叶云峰 张晋 付岗 |
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| 作者单位： | 1. 广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室, 南宁 530007;2. 广西农业科学院园艺研究所, 南宁 530007 |
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| 基金项目： | 国家自然科学基金（31560006）；广西自然科学基金（2017GXNSFDA198002）；广西创新驱动发展专项（桂科AA18118028-5）；广西农业科学院科技发展基金（桂农科2018YT15，31960520）；广西作物病虫害生物学重点实验室基金（2019-ST-03）。 |
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| 摘 要： | 为了进一步改良拮抗菌在生物菌肥中的生存条件，提高香蕉枯萎病生防菌肥的防病促生效果，以GFP标记的拮抗木霉GFP-gz-2为指示菌，对生物肥中与拮抗菌菌株生长相关的多种因子进行了优化，并进一步评价了菌肥优化前后的防病促生效果。试验结果表明，在菌肥的7种待添加碳源中，糯米粉为菌株GFP-gz-2的最佳碳源，当糯米粉的含量为35 g/L时，其菌落直径和产孢量达到最大值；在菌肥成分的6种金属离子中，Ca2+最利于菌株GFP-gz-2的生长。多因素正交优化结果表明，适于菌株GFP-gz-2生长的生物肥最佳配制条件为有机肥的C/N为30：1，Ca2+浓度为5%，菌株的接种量为2.0×106孢子/g。在该条件下，菌株GFP-gz-2在生物肥中的定殖数量为1.033×108孢子/g。改良菌肥对香蕉枯萎病的防治效果达到84.75%，比优化前防效提高15.26%。改良菌肥可显著提高香蕉的株高、茎围、鲜重及干重，比优化前分别提高23.35%、22.82%、68.78%和50.17%，具有明显的促生作用。
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| 关 键 词： | 香蕉枯萎病 哈茨木霉 菌肥 配方优化 |
| 收稿时间： | 2019-10-11 |
Bio-Fertilizer Optimization and Its Control Effect on Banana Fusarium Wilt |
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| DU Chanjuan,YANG Di,PAN Lianfu,YE Yunfeng,ZHANG Jin,FU Gang.Bio-Fertilizer Optimization and Its Control Effect on Banana Fusarium Wilt[J].Chinese Journal of Biological Control,2020,36(3):396-404. |
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| Authors: | DU Chanjuan YANG Di PAN Lianfu YE Yunfeng ZHANG Jin FU Gang |
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| Affiliation: | 1. Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests/Institute of Plant Protection Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;2. Institute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning 530007, China |
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| Abstract: | In order to improve the living conditions of antagonistic fungus in the fertilizer and improve control effect of the bio-fertilizer on banana Fusarium wilt, a variety of factors related to the growth of antagonistic strain were optimized based on the GFP-labeled strain Thichoderma harzianum GFP-gz-2. The effects of the bio-fertilizer on disease control and growth promotion were evaluated before and after optimization, respectively. The results showed that glutinous rice flour was the best one among the 7 carbon sources for the growth of strain GFP-gz-2. The colony diameter and sporulation of strain GFP-gz-2 reached the maximum when the content of glutinous rice flour was 35 g/L. Ca2+ was the best one in the 6 metal ions for the growth of strain GFP-gz-2. The results of multi-factor orthogonal optimization showed that the best conditions for the growth of strain GFP-gz-2 in the bio-fertilizer were listed as follows:C/N ratio of organic fertilizer was 30:1, concentration of Ca2+was 5%, and the inoculation amount of the strain was 2.0×106 spore/g. Under these conditions, the colonization amount of strain GFP-gz-2 in the bio-fertilizer was 1.033×108 spore/g. The control effect of optimized bio-fertilizer on banana Fusarium wilt reached to 84.75%, 15.26% higher than that before optimization. The optimized bio-fertilizer increased the plant height, stem girth, fresh weight and dry weight of banana significantly, which were 23.35%, 22.82%, 68.78% and 50.17% higher than that before optimization, respectively. |
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| Keywords: | banana Fusarium wilt Trichoderma harzianum fungus fertilizer formulation optimization |
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