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中国荷斯坦成年公牛可消化有机物进食量与尿嘌呤衍生物排出量的关系分析
引用本文:徐萍,莫放,姜军,高博.中国荷斯坦成年公牛可消化有机物进食量与尿嘌呤衍生物排出量的关系分析[J].中国农学通报,2007,23(2):1-4.
作者姓名:徐萍  莫放  姜军  高博
作者单位:中国农业大学动物营养学国家重点实验室,100094,北京
基金项目:基金项目:农业部十五科技攻关“尿中嘌呤衍生物法估测瘤胃微生物蛋白质产生量”(2002BA514-10-2-3).
摘    要:尿液嘌呤衍生物法作为一种估测瘤胃微生物蛋白质合成量的新方法,克服了原有方法的缺点,简单易行,通过欧洲牛羊的实验证明该方法与其它方法的测定结果有很好的一致性,但此方法亦有其不足。由于尿中嘌呤衍生物排出量受动物品种的影响很大,不同品种间无通用性,所以现有的研究成果不能用于中国品种牛,更难以使该方法应用于生产。而国内在此方面的报道很有限,所以急需建立各品种反刍动物的估测模型,扩大该方法的应用范围。试验采用3头月龄(22±0.2),体重为(641±26.889)kg,体况相近的中国荷斯坦成年公牛,设计3×3的拉丁方,通过调整日粮精粗比来考察可消化有机物进食量对中国荷斯坦成年公牛尿嘌呤衍生物(PD)排出的影响。结果发现,试验牛尿中嘌呤衍生物中尿囊素大约为73% ̄88%,黄嘌呤和次黄嘌呤的含量很小,可以忽略不计,与以往对成年反刍动物的研究结果相似,与同个体犊牛期的研究结果相反。PD的排出量随可消化有机物(DOMI)和可消化粗蛋白(DCP)进食量升高而上升,存在较强的相关性(R2=0.88,n=9),其排出水平为414.09 ̄975.59mmol/d。肌酐酸的排出较稳定,各处理间差异不显著(P>0.05)。PDC与DOM和DCP进食量存在正相关,这与以往成年反刍动物的研究结果是相似的,与同个体犊牛期的研究结果相反,但相关性较弱。

关 键 词:可消化有机物  嘌呤衍生物  成年牛
修稿时间:2006-04-07

The Relationship between Digestible Organic Matter and Purine Derivatives in Urine in Grown Male Cattle of China Holstein
Xu Ping,Mo Fang,Jiang Jun,Gao Bo.The Relationship between Digestible Organic Matter and Purine Derivatives in Urine in Grown Male Cattle of China Holstein[J].Chinese Agricultural Science Bulletin,2007,23(2):1-4.
Authors:Xu Ping  Mo Fang  Jiang Jun  Gao Bo
Affiliation:College of Animal Science and Technology, China Agricultural University, Beijing 100094
Abstract:As one of the way of estimating microbial protein production, urinary purine derivatives(PD) is easier. And it has good consistency with any other ways, but it also has disadvantage. The PD excretion in urine changes and it is not universal in different breeds. So it is exactly in need of kinds of models about different ruminants. Three China Holstein male cattle were fed with three mixed diets of forage and concentrate. In this experiment, a Latin square design was used to research the rules of purine derivatives excretion through observing the relationship between DOMI and PD excretion. It shows that the allantoin ,which only takes little part in calves, was the main part (about73%~88%) in the cattle's urine, and the xanthine and hypoxanthine which are also take only much little part, can be neglected. The PD changed from 414.09mmol/d to 975.59mmol/d with the enhancement of DOM and DCP intake, and the relativity was significant (R2=0.88, n=9). At the same time, the creatine excretion was steady, and not significant(P>0.05). The PDC increased with the enhancement of DOM and DCPI, which was consistent to other's research of cattle and contrary to calves, but the response was lower.
Keywords:Digestible organic matter  Purine derivatives  Cattle
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