| 金龟子绿僵菌FM-03生物学特性及其对柑橘粉蚧的侵染 |
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| 引用本文： | 黄鹏,姚锦爱,余德亿.金龟子绿僵菌FM-03生物学特性及其对柑橘粉蚧的侵染[J].中国生物防治学报,2018,34(6):858-865. |
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| 作者姓名： | 黄鹏 姚锦爱 余德亿 |
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| 作者单位： | 1. 福建省农业科学院植物保护研究所, 福州 350013;2. 福建省作物有害生物监测与治理重点实验室, 福州 350003 |
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| 基金项目： | 福建省省属公益类科研院所基本科研专项（2016R1023-8，2018R1025-2）；福建省农业引导性（重点）项目（2017N0063）；福建省农业科学院一般科研项目（AC2017-12）；福建省农业科学院科技创新团队建设项目（STTT2017-2-2） |
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| 摘 要： | 为明确金龟子绿僵菌FM-03菌株生物学特性及其对柑橘粉蚧的侵染效果，室内测定不同温度、pH、光周期、碳氮源对该菌株生长和产孢量的影响；观察柑橘粉蚧受该菌株侵染后的死亡趋势，构建该菌株对柑橘粉蚧的时间-剂量-死亡率（TDM）模型。结果表明，金龟子绿僵菌FM-03菌株培养最适的温度为25℃、pH 7、光周期12L：12D、碳源为乳糖、氮源为酵母膏，在此培养条件下菌落直径和产孢量分别可达6.17 cm和3.70×109孢子/皿。柑橘粉蚧受菌株FM-03侵染后的死亡趋势随菌株浓度升高和侵染时间延长而上升，1×108、1×107和1×106孢子/mL处理的升幅明显高于其他2个处理，其中在1×108孢子/mL处理的累计校正死亡率最高可达82.02%；构建的TDM模型，通过Pearson卡方和Hosmer-Lemeshow检验，显示菌株FM-03对柑橘粉蚧的剂量效应随侵染时间的延长逐渐增强，时间效应随浓度的升高逐渐增强，侵染8 d后的LC50和1.00×108孢子/mL处理的LT50分别可达6.03×105孢子/mL和4.53 d。综上，金龟子绿僵菌FM-03菌株生长快、产孢量高，对柑橘粉蚧的致病力强，生防应用前景良好。
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| 关 键 词： | 金龟子绿僵菌 生物学特性 柑橘粉蚧 时间-剂量-死亡率模型 |
| 收稿时间： | 2018-06-22 |
Biological Characteristics of Metarhizium anisopliae FM-03 and Its Infection against Planococcus citri |
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| HUANG Peng,YAO Jinai,YU Deyi.Biological Characteristics of Metarhizium anisopliae FM-03 and Its Infection against Planococcus citri[J].Chinese Journal of Biological Control,2018,34(6):858-865. |
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| Authors: | HUANG Peng YAO Jinai YU Deyi |
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| Affiliation: | 1. Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;2. Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou 350003, China |
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| Abstract: | To definite the biological characteristics of Metarhizium anisopliae strain FM-03, the effects of temperature, pH, photoperiod, carbon source and nitrogen source on the mycelia growth and sporulation of FM-03 strain were tested. Then the mortality of FM-03-infected Planococcus citri was observed, and the time-dose-mortality model was constructed. Results showed that the optimal culture conditions for FM-03 growth and sporulation were 25℃, pH 7, 12L:12D, with lactose as carbon source and yeast extract as nitrogen source. With these conditions, the colony diameter and sporulation quantity of FM-03 were 6.17 cm and 3.70×109 conidia/dish, respectively. The mortality of FM-03-infected P. citri increased with the increase in conidial concentration and the time of infection. The mortality increased more at 1×108, 1×107 and 1×106 conidia/mL than at 1×105 and 1×104 conidia/mL. The highest corrected accumulative mortality of P. citri reached 82.02% at 1×108 conidia/mL. Through Pearson's chi-squared test and Hosmer-Lemeshow test, the TDM model of FM-03 against P. citri showed an enhanced dose effect of FM-03 against P. citri with the prolong of infection time, and an improved time effect with the increase in conidial concentration. The LC50 of FM-03 reached 6.03×105 conidia/mL after an eight-day infection. The LT50 of 1.00×108 conidia/mL reached 4.53 d. In conclusion, with fast growth, high sporulation quantity, and strong pathogenicity against P. citri, M. anisopliae FM-03 shows an excellent potential in biological control of the pest. |
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| Keywords: | Metarhizium anisopliae biological characteristics Planococcus citri time-dose-mortality model |
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