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Effects of different forage combinations in total mixed rations on in vitro gas production kinetics,ruminal and milk fatty acid profiles of lactating cows
Authors:Sarvvl Bai  Zhi‐Jun Cao  Bin‐Bin Cao  Hong‐Jian Yang  Sheng‐Li Li  Jian‐Xin Liu
Affiliation:1. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China;2. Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, China;3. Institute of Dairy Science, MoE Key laboratory of Molecular Animal Nutrition, College of Animal Science, Zhejiang University, Hangzhou, China
Abstract:This study aimed to determine the effects of different forage combinations on in vitro gas production (GP) kinetics, ruminal and milk fatty acid profiles. Forty‐five lactating cows were randomly arranged into three groups and fed three total mixed rations (TMRs) with different forage combinations: TMR1, 23% alfalfa hay, 7% Chinese wild ryegrass hay and 15% whole corn silage; TMR2, 30% corn stover plus 15% whole corn silage; TMR3, 30% rice straw plus 15% whole corn silage. In vitro dry matter disappearance ranked: TMR1 > TMR2 > TMR3, and highest cumulative GP and asymptotic GP occurred in TMR1 while no difference occurred between TMR2 and TMR3. The average GP rate ranked: TMR1 > TMR2 > TMR3. TMR1 in comparison with TMR2 and TMR3 presented lower rumen contents of acetate and butyrate and greater rumen contents of propionate, valerate, C13:0, C14:0, C15:0, C18:1cis‐9, C18:2n‐6, C18:3n‐3, C20:0 and C22:0 as well as milk C18:2n‐6 and C18:3n‐3 proportions. Transfer efficiencies of C18:2n‐6 and C18:3n‐3 from diet to milk ranked: TMR1 > TMR2 > TMR3. The findings suggest TMRs containing alfalfa hay and Chinese wild ryegrass hay in comparison with corn stover or rice straw improve rumen fermentation and transfer efficiency of C18:2n‐6 and C18:3n‐3.
Keywords:forage combination  kinetic gas production  lactating cow  milk fatty acid  ruminal fatty acid
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