|
|||||
|
|
| 鸡源致病性奇异变形杆菌的分离鉴定与遗传进化分析 | |
| 引用本文： | 徐睿,李颜桃. 鸡源致病性奇异变形杆菌的分离鉴定与遗传进化分析[J]. 中国畜牧兽医, 2018, 45(9): 2550-2558. DOI: 10.16431/j.cnki.1671-7236.2018.09.027 |
| 作者姓名： | 徐睿 李颜桃 |
| 作者单位： | 1. 西昌学院动物科学学院, 西昌 615000; 2. 攀西动物疫病检测与防控高校重点实验室, 西昌 615013 |
| 基金项目： | 四川省凉山州农业科技创新项目（15NYCX0040）；四川省科技厅支撑计划项目（13ZC1796）；四川省教育厅攀西动物疫病防控与检测高校重点实验室（纵341B） |
| 摘 要： | 为了解鸡源奇异变形杆菌的耐药情况、致病力和遗传特征，本试验从凉山地区分离得到2株细菌（YLF1、WS），通过培养特征、镜检特征和16S rRNA序列测定对分离菌进行鉴定及致病性试验；采用（K-B）法检测分离菌对14种常用抗生素（包括β-内酰胺类、头孢烯类、氨基糖苷类、四环素类、氟喹诺酮类、大环内酯类）的敏感性；并与GenBank中公布的21种不同来源的变形杆菌16S rRNA基因序列进行遗传进化分析。结果表明，YLF1、WS菌株均具有迁徙性和β-溶血生长特征，镜检为革兰氏阴性长短不一的杆菌或球菌，经16S rRNA基因鉴定与GenBank中奇异变形杆菌同源性均在99%以上，YLF1、WS菌株确定为奇异变形杆菌，均表现为多重耐药，耐药率分别为78.6%（11/14）和71.4%（10/14）；雏鸡致死率均为80%；YLF1、WS菌株与日本人体分离株、中国新疆黄牛分离株和中国广东土壤分离株同源性最高，达99.9%；与来自中国河南、中国新疆及印度的鸡源奇异变形杆菌同源性较高（98.9%~99.3%），遗传关系密切。表明凉山地区鸡群中存在奇异变形杆菌感染，分离株有较高的致病力和较强的耐药性，也提示分离菌可能来源于环境、感染鸡或其他动物，同时存在人畜共患的潜在危险。 |
| 关 键 词： | 鸡 奇异变形杆菌 分离鉴定 遗传进化分析 |
| 收稿时间： | 2018-03-16 |
Isolation,Identification and Genetic Evolution Analysis of Chicken Pathogenic Proteus mirabilis |
|
| XU Rui,LI Yantao. Isolation,Identification and Genetic Evolution Analysis of Chicken Pathogenic Proteus mirabilis[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(9): 2550-2558. DOI: 10.16431/j.cnki.1671-7236.2018.09.027 | |
| Authors: | XU Rui LI Yantao |
| Affiliation: | 1. Department of Animal Science, Xichang University, Xichang 615000, China; 2. Key Laboratory for Control and Prevention of Animal Blight in Panxi, Xichang 615013, China |
| Abstract: | In order to understand the drug resistance,virulence and genetic characteristics of chicken source Proteus mirabilis,2 strains of bacteria (YLF1 and WS strains) were prepared by isolation from the Liangshan area,and they were identified by culture characteristics,microscopic characteristics and 16S rRNA sequence determination,and pathogenicity test.Then the sensitivity of the bacteria to 14 commonly used antibiotics (including β-lactams,ceftenes,aminoglycosides,tetracyclines,fulro quinolones and macrolides) was determined using the (K-B) method;The phylogenetic analysis of the 16S rRNA gene sequences of Proteus from 21 different sources published in GenBank was carried out.The results showed that the YLF1 and WS strains all had migratory and β-hemolytic growth characteristics.The microscopic examination showed gram-negative bacilli or cocci varied in length,the homology of them with Proteus mirabilis in GenBank through the 16S rRNA gene identification was above 99%,YLF1 and WS strains were identified as Proteus mirabilis;YLF1 and WS strains showed multiple drug resistance,the drug resistance rates were 78.6%(11/14) and 71.4%(10/14),respectively,the mortality rate of chicks was 80%,the YLF1 and WS strains had the highest homology with Japanese isolate from human,Xinjiang isolate from cattle and Guangdong isolate from soil (99.9%),and they were highly homologous to chicken source Proteus mirabilis from Henan,Xinjiang and India (98.9% to 99.3%),and the genetic relationship was close.These results indicated that Proteus mirabilis infection existed in the chicken populations of Liangshan area.The isolates had high virulence and strong resistance,it also suggested that the isolates might be from the environment,infected chickens or other animals,and there was a potential risk of zoonotic diseases. |
| Keywords: | chicken Proteus mirabilis isolation and identification genetic evolution analysis |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 | |
| 点击此处可从《中国畜牧兽医》下载全文 | |