| TaCYP78A5基因过表达小麦的遗传和功能初步分析 |
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| 引用本文： | 陈之忍,马 猛,申玉霞,吴林楠,刘香利,赵惠贤.TaCYP78A5基因过表达小麦的遗传和功能初步分析[J].麦类作物学报,2017(6):721-729. |
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| 作者姓名： | 陈之忍 马 猛 申玉霞 吴林楠 刘香利 赵惠贤 |
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| 作者单位： | 1. 西北农林科技大学生命科学学院,陕西杨陵,712100;2. 西北农林科技大学生命科学学院,陕西杨陵 712100;西北农林科技大学旱区作物逆境生物学国家重点实验室,陕西杨陵 712100 |
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| 基金项目： | 国家自然科学基金项目（31471482，31101205）;西北农林科技大学基本科研业务费专项（Z109021565，Z109021568） |
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| 摘 要： | 为了验证小麦籽粒大小相关基因TaCYP78A5在小麦籽粒发育中的功能,对pINO启动子驱动的TaCYP78A5基因过表达的转基因小麦后代株系进行了鉴定,检测了T_0代植株目标基因拷贝数,定量分析了7个T_1代阳性植株的目标基因表达,并对其籽粒大小进行了统计。结果表明,利用Bar试纸条和目标基因特异PCR检测相结合的方法对21株转基因T_0代再生苗进行检测,共鉴定出14个阳性植株,除2个植株的目标基因拷贝数为3和1个植株为7外,其余11个T_0代转基因植株目标基因插入拷贝数均为1~2个,其中有6个单拷贝植株。与野生型相比,7个T_1代阳性植株目标基因表达量均极显著增加,粒厚和粒宽均有不同程度增加,粒重极显著增加。
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| 关 键 词： | 小麦 TaCYP78A5基因 Bar基因 转基因株系 |
Genetic and Functional Analysis of Transgenic Wheat Overexpressing TaCYP78A5 Gene |
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| CHEN Zhiren,MA Meng,SHEN Yuxi,WU Linnan,LIU Xiangli,ZHAO Huixian.Genetic and Functional Analysis of Transgenic Wheat Overexpressing TaCYP78A5 Gene[J].Journal of Triticeae Crops,2017(6):721-729. |
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| Authors: | CHEN Zhiren MA Meng SHEN Yuxi WU Linnan LIU Xiangli ZHAO Huixian |
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| Abstract: | In order to verify the function of TaCYP78A5 gene,which is associated with wheat (Triticum aestivum L.) grain size in the process of wheat grain development,the transgenic wheat lines with the TaCYP78A5 gene overexpression under the controlling of pINO promoter were used as materials. The copy number of target gene in T0 generation transgenic plants was detected.The expression of the target gene of seven T1 positive plants was tested and the grain size were measured. The results showed that out of 21 regenerated seedlings,14 positive plants were obtained by combining Bar strip test and PCR detection. The results of copy number detection showed that two and one transgenic plants had three and seven copies of target gene,respectively,and the rest eleven transgenic plants each had a copy number of 1 or 2,among which there were six single copy plants.Compared with the wild type JW1,the expression levels of the target gene in seven T1 positive plants were significantly increased,the grain width and grain thickness were increased by different rates,and the grain weight were significantly increased. |
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| Keywords: | Wheat TaCYP78A5 gene Bar gene Transgenic lines |
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