Breeding for boron tolerance in lentil (Lens culinaris Medik.) using a high‐throughput phenotypic assay and molecular markers |
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| Authors: | Matthew S. Rodda Shimna Sudheesh Muhammad Javid Dianne Noy Annathurai Gnanasambandam Anthony T. Slater Garry M. Rosewarne Sukhjiwan Kaur |
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| Affiliation: | 1. Agriculture Victoria Research, Grains Innovation Park, Horsham, Vic., Australia;2. Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Vic., Australia;3. Plant Genetic Solutions Pty Ltd, Redbank Plains, Qld, Australia |
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| Abstract: | This study describes the identification of a quantitative trait locus (QTL) in the recombinant inbred line population of ILL2024 × ILL6788 and subsequent validation of associated molecular markers. A high‐quality genetic linkage map was constructed with 758 markers that cover 1,057 cM, with an average intermarker distance of 2 cM. QTL analysis revealed a single genomic region on Lc2 to be associated with B tolerance and accounted for up to 76% of phenotypic variation (Vp). The best markers for B tolerance were assessed for their utility in routine breeding applications using validation panels of diverse lentil germplasm and breeding material derived from ILL2024. A marker generated from the dense genetic map of this study was found to be the most accurate of all markers available for B tolerance in lentil, with a success rate of 93% within a large breeding pool derived from ILL2024. However, given the number of the unrelated lines for which the marker–trait association was not conserved, B tolerance screening is still required at later stages to confirm predicted phenotypes. |
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| Keywords: | abiotic stress tolerance marker‐assisted selection molecular markers plant breeding |
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