| 不同浓度NaCl对‘达米娜’葡萄影响的主成分分析 |
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| 引用本文： | 谭 伟,李晓梅,董志刚,谭 敏,茹慧玲,唐晓萍.不同浓度NaCl对‘达米娜’葡萄影响的主成分分析[J].农学学报,2017,7(9):42-47. |
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| 作者姓名： | 谭 伟 李晓梅 董志刚 谭 敏 茹慧玲 唐晓萍 |
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| 作者单位： | 山西省农业科学院果树研究所/果树种质创制与利用山西省重点实验室,山西太谷,030815 |
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| 基金项目： | 山西省农业科学院农业科技创新研究课题“盐胁迫下外源钙调节葡萄活性氧代谢的机理”(ZDSYS1505)；山西省重点研发计划重点项目 “葡萄产业提质增效关键技术研发与示范”(201603D21105)。 |
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| 摘 要： | 为筛选NaCl胁迫对葡萄影响的主要指标,建立评价葡萄抗盐性体系提供理论依据,以沙培1年生‘达米娜’葡萄自根苗为试验材料,研究6个浓度NaCl胁迫10天后,葡萄根系和叶片相对电导率、叶片超氧阴离子产生速率、H_2O_2含量、抗氧化酶(SOD、CAT、POD、APX)活性、植株新梢长度和总生物量的变化。结果表明:与对照相比,随着NaCl浓度的升高,根系和叶片相对电导率呈逐渐上升趋势,叶片超氧阴离子产生速率和H_2O_2含量增加,叶片中SOD和CAT活性呈先升高后降低的趋势,APX活性呈现"降低—升高—降低"的趋势,而POD活性呈下降的趋势;高浓度NaCl胁迫下,新梢长度和植株总生物量降低,叶片超氧阴离子产生速率和H_2O_2含量增加可能与较低的抗氧化酶活性有关。通过主成分分析得到,新梢长度、叶片CAT活性、H_2O_2含量和APX活性可作为不同浓度NaCl胁迫对葡萄影响的评价指标。
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| 关 键 词： | 葡萄 盐胁迫 主成分分析 |
| 收稿时间： | 2017/6/19 0:00:00 |
| 修稿时间： | 2017/6/30 0:00:00 |
Principal Component Analysis of the Effect of Different NaCl Concentrations on'Tamina'Grape Seedlings |
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| Tan Wei,Li Xiaomei,Dong Zhigang,Tan Min,Ru Huiling,Tang Xiaoping.Principal Component Analysis of the Effect of Different NaCl Concentrations on'Tamina'Grape Seedlings[J].Journal of Agriculture,2017,7(9):42-47. |
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| Authors: | Tan Wei Li Xiaomei Dong Zhigang Tan Min Ru Huiling Tang Xiaoping |
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| Abstract: | The paper aims to select the main indexes of NaCl stress on grapevine and provide a theory basis for evaluation the salt resistance system of grapevines. With annual grape'Tamina'grown in sand as the experimental material, six concentrations of NaCl were conducted, the relative conductivity of grape root and leaf, the oxygen anion production rate, H2O2 content, antioxidant enzyme (SOD, CAT, POD, APX) activities of leaves, shoot length and total biomass of plants were analyzed. Results showed that the root and leaf relative conductivity increased gradually with the increase of NaCl concentration compared with the control, the super oxygen anion production rate and H2O2 content increased, SOD and CAT activity in the leaves presented a trend of rise-down, APX activity presented a trend of down-rise-down, while POD activity presented a downward trend; under high concentrations of NaCl, the length of shoot and total biomass of plant were reduced, higher oxygen anion production rate and H2O2 content in grape leaves might be related with the lower antioxidant enzyme activity. The principal component analysis indicated that the shoot length, CAT activity, H2O2 content and APX activity of leaves could be used to evaluate the effects of different concentratio ns of NaCl stress on grapevine. |
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| Keywords: | grape NaCl stress Principal component analysis |
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