| 北美豆梨组培快繁技术研究 |
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| 引用本文： | 邱玉宾,康晓瑜,杨志莹,李田,赵庆柱.北美豆梨组培快繁技术研究[J].农学学报,2015,5(12):64-69. |
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| 作者姓名： | 邱玉宾 康晓瑜 杨志莹 李田 赵庆柱 |
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| 作者单位： | 山东省潍坊市农业科学院,高密市林业局,山东省潍坊市农业科学院,山东省潍坊市农业科学院,山东省潍坊市农业科学院 |
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| 基金项目： | 潍坊市科技攻关项目“北美豆梨引种驯化及繁育技术研究”(2010-1001)。 |
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| 摘 要： | 为探讨北美豆梨组培快繁技术体系，以引进的北美豆梨‘首都’为试验材料，进行不同灭菌时间、不同基本培养基、增殖及生根培养、移栽基质的选择等试验。结果表明，灭菌14 min 为5 种灭菌时间中最佳灭菌时间，其成活率虽略低于灭菌16 min，但外植体萌芽快，长势正常；MS培养基为北美豆梨理想的基本培养基；MS添加0.5 mg/LTDZ+0.2 mg/LNAA+0.05 mg/LGGR6+琼脂粉6.0 g/L+蔗糖30 g/L+活性炭2.0 g/L为豆梨的最适增殖培养基配方；最佳生根培养基配方为MS+TDZ 0.5 mg/L+ABT1#1.5 mg/L+蔗糖20 g/L+琼脂粉4.5 g/L+活性炭2.0 g/L；草炭+珍珠岩（比例2:1）作为栽培基质移栽成活率最高，生长表现最好，幼苗健壮，叶色深绿。
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| 关 键 词： | 最低气温 最低气温 国家级观测站与自动气象站对比 气温差异 |
| 收稿时间： | 2015/2/3 0:00:00 |
| 修稿时间： | 2015/5/20 0:00:00 |
Study on Tissue Culture and Rapid Propagation Technique of Pyrus calleryana Decne |
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| Abstract: | In order to investigate a rapid propagation cultivation technique system of Pyrus calleryana Decne, Pyrus calleryana Decne‘Capital’was used as experimental materials. Different sterilization time, different basic culture media, proliferation culture and tube seedlings rooting culture, and the choosing of cultivation medium were studied. The results showed that 14 min was the best length of 5 different times in the sterilization process, although the rate of survival was lower than that of 16 min, but the explants sprouted quickly and grew normally. MS was the basic culture medium. Adding 0.5 mg/LTDZ, 0.2 mg/L NAA, 0.05 mg/L GGR6, 6.0 g/L AGAR powder, 30 g/L sucrose, 2.0 g/L activated carbon to MS was the optimal proliferation medium formula. Adding 0.5 mg/L TDZ, 1.5 mg/L ABT1#, 20 g/L sucrose, 4.5 g/L AGAR powder, 2.0 g/L activated carbon to MS was the optimal rooting medium formula. Transplanting survival rate was the highest, and the plants had the best growth performance, robust growing, and dark green leaf color when peat and perlite (a ratio of 2:1) were used as the cultivation medium. |
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| Keywords: | Pyrus calleryana Decne arth rapid propagation TDZ ABT1# GGR6 browning |
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