| 高繁殖力和低繁殖力长白公猪的精子DFI差异分析 |
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| 引用本文： | 杨凯琳,胡家豪,司景磊,陈献欣,郑启源,田奎,张浪,陆阳清,许常龙.高繁殖力和低繁殖力长白公猪的精子DFI差异分析[J].南方农业学报,2022,53(11):3229-3236. |
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| 作者姓名： | 杨凯琳 胡家豪 司景磊 陈献欣 郑启源 田奎 张浪 陆阳清 许常龙 |
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| 作者单位： | 1. 广西大学动物科学技术学院, 广西南宁 530004;2. 广西农垦永新畜牧集团有限公司, 广西南宁 530300;3. 南宁市第二人民医院生殖医疗中心, 广西南宁 530031 |
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| 基金项目： | 广西自然科学基金项目(2020GXNSFAA159099)广西畜牧产业科技先锋队(桂农科盟202209-02) |
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| 摘 要： | 【目的】探究高繁殖力和低繁殖力长白公猪的精液品质及精子DNA损伤差异,为筛选出高繁殖力种公猪提供参考依据,进而优化猪场人工授精工作及提高种猪场的经济效益。【方法】选取12头法系长白公猪,通过分析繁育数据将其分组为高繁殖力和低繁殖力公猪,各6头;利用计算机辅助精子分析系统(CASA)对公猪精液品质参数进行检测分析,然后通过流式细胞仪检测精子DNA碎片指数(DFI)和活性氧(ROS)水平。【结果】高繁殖力公猪的平均窝产仔数和活产仔数均极显著高于低繁殖力公猪(P<0.01,下同),但二者的畸胎数和死胎数差异不显著(P>0.05,下同)。高繁殖力公猪精子的前进运动率显著高于低繁殖力公猪精子(P<0.05,下同),而精子畸形率极显著低于低繁殖力公猪,但二者在近端原生质滴率、远端原生质滴率及折尾率等方面的差异均不显著;除了高繁殖力公猪精子的曲线速率(VCL)显著高于低繁殖力公猪外,其他的运动参数均无显著差异;高繁殖力公猪的精子DFI和ROS水平均极显著低于低繁殖力公猪。长白公猪产仔数与各项精液质量参数的相关分析发现,公猪产仔数与精子DFI呈高度负相关(r=-0.85)、与精子畸形率(r=-0.70)和精子ROS水平(r=-0.78)呈中度负相关;公猪精子畸形率与精子DFI(r=0.63)和ROS水平(r=0.62)呈中度正相关;精子DFI和ROS水平呈高度正相关(r=0.93)。【结论】精子DNA碎片化与公猪繁殖力密切相关,即精子DFI可为评估公猪精液品质及繁殖力提供重要参考依据。因此,在筛选种公猪时精子DFI检测可作为公猪精子质量评估的补充手段,以协同人工授精技术提高受精率及猪场经济效益,进而推动养猪业的高质量发展。
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| 关 键 词： | 长白公猪 精液品质 精子DFI 产仔数 |
| 收稿时间： | 2022-05-24 |
Differential analysis of sperm DFI in Landrace boars with high and low reproductive performance |
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| YANG Kai-lin,HU Jia-hao,SI Jing-lei,CHEN Xian-xin,ZHENG Qi-yuan,TIAN Kui,ZHANG Lang,LU Yang-qing,XU Chang-long.Differential analysis of sperm DFI in Landrace boars with high and low reproductive performance[J].Journal of Southern Agriculture,2022,53(11):3229-3236. |
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| Authors: | YANG Kai-lin HU Jia-hao SI Jing-lei CHEN Xian-xin ZHENG Qi-yuan TIAN Kui ZHANG Lang LU Yang-qing XU Chang-long |
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| Affiliation: | 1. College of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530004, China;2. Guangxi State Farms Yongxin Animal Husbandry Group Co., Ltd., Nanning, Guangxi 530300, China;3. Reproductive Medical Center of the Second Nanning People's Hospital, Nanning, Guangxi 530031, China |
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| Abstract: | 【Objective】This experiment was aimed to investigate the differences of semen quality and sperm DNA damage in Landrace boars with high and low reproductive performance,provide reference basis for screening out high fertility boars,so as to optimize artificial insemination ofpigs and improve the economic benefits of pig farms.【Method】Twelve French landrace boars were selected and divided into two groups of high and low reproductive performance by analyzing breeding data,with 6 pigs in each group. The boar semen quality parameters were determined by computer-aided sperm analysis(CASA) system. The sperm DNA fragmentation index(DFI) and reactive oxygen species(ROS) level were determined by flow cytometer.【Result】The average litter size and alive litter size of high fertility boars were extremely significantly higher than those of low fertility boars(P<0.01,the same below),but there was no significant difference in abnormal litter size and dead litter size between the two groups(P>0.05,the same below). The sperm progressive movement rate of high fertility boars was significantly higher than that of low fertility boars(P<0.05,the same below),and the sperm deformity rate was extremely significantly lower than that of low ones,but there was no significant difference between the two in the rate of proximal protoplasm drop,distal protoplasm drop and abnormal tail. Except that the curvilinear velocity(VCL) of spermatozoa of high fertility boars was significantly higher than that of low fertility boars,there was no significant difference in other motion parameters. The levels of sperm DFI and ROS in high fertility boars were extremely significantly lower than those in low fertility group. The correlation analysis between litter size and semen quality parameters of Landrace boars showed that litter size was highly negatively correlated with sperm DFI(r=-0.85),moderately negatively correlated with sperm deformity rate(r=-0.70) and sperm ROS level(r=-0.78),and the sperm deformity rate of Landrace boars was positively correlated with sperm DFI(r=0.63) and ROS level(r=0.62). Moreover,there was a highly positive correlation between sperm DFI and ROS levels(r=0.93).【Conclusion】Sperm DNA fragmentation is closely related to boar reproductive performance,sosperm DFI can provide an important reference for evaluating boar semen quality and reproductive performance. Therefore,the detection of sperm DFI during the selection of boar sperm collection can be used as a supplementary method for the evaluation of boar sperm quality,so as to improve the fertilization rate and the economic benefits of the pig farm in cooperation with artificial insemination technology,thus promoting the high-quality development of the pig industry. |
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