| 木奶果种质资源的遗传多样性分析及DNA指纹图谱构建 |
| |
| 引用本文： | 周彩霞,罗培四,孔方南,蒋娟娟,卓福昌,李文砚,周婧,韦优.木奶果种质资源的遗传多样性分析及DNA指纹图谱构建[J].南方农业学报,2022,53(5):1207-1215. |
| |
| 作者姓名： | 周彩霞 罗培四 孔方南 蒋娟娟 卓福昌 李文砚 周婧 韦优 |
| |
| 作者单位： | 广西农业科学院广西南亚热带农业科学研究所, 广西崇左 532415 |
| |
| 基金项目： | 中央引导地方科技发展专项（桂科ZY19183010）；广西农业科学院科技发展基金项目（桂农科2020YM150）；广西农业科学院基本科研业务专项（桂农科2021YT163） |
| |
| 摘 要： | 【目的】对来自广西、云南、海南和广东的63份木奶果种质资源进行遗传多样性分析,并构建对应的DNA指纹图谱，为木奶果种质资源的分类鉴定、保护和利用提供理论参考。【方法】选取3份地理位置相差较远的种质，从75条SCoT引物中筛选出条带清晰、重复性好和多态性丰富的引物，对63份供试种质进行多态性扩增，利用Popgene 1....
|
| 关 键 词： | 木奶果 种质资源 SCoT分子标记 遗传多样性 DNA指纹图谱 |
| 收稿时间： | 2022-02-02 |
Genetic diversity analysis and DNA fingerprinting construction with Baccaurea ramiflora Lour. germplasm resources |
| |
| ZHOU Cai-xia,LUO Pei-si,KONG Fang-nan,JIANG Juan-juan,ZHUO Fu-chang,LI Wen-yan,ZHOU Jing,WEI You.Genetic diversity analysis and DNA fingerprinting construction with Baccaurea ramiflora Lour. germplasm resources[J].Journal of Southern Agriculture,2022,53(5):1207-1215. |
| |
| Authors: | ZHOU Cai-xia LUO Pei-si KONG Fang-nan JIANG Juan-juan ZHUO Fu-chang LI Wen-yan ZHOU Jing WEI You |
| |
| Affiliation: | Guangxi South Subtropical Agricultural Science Research Institute, Guangxi Academy of Agricultural Sciences, Chongzuo, Guangxi 532415, China |
| |
| Abstract: | 【Objective】To conduct genetic diversity analysis of 63 Baccaurea ramiflora Lour. germplasm resources from Guangxi，Yunnan，Hainan and Guangdong and to establish a preliminary DNA fingerprint，so as to provide a reference for varieties classification，identification，protection，and utilization of B. ramiflora Lour.【Method】Three germplasm resources whose geographical locations were highly different were selected，and the SCoT primers with best quality，repeatability and polymorphism were screened from 75 SCoT primers to perform amplification and electrophoresis on 63 germplasm resources. Genetic diversity and clustering analysis were performed by Popgene 1.32 and NTsys 2.10e and the results were used to construct the fingerprint profiles.【Result】The 6 SCoT primers selected from 63 germplasm resources were used to amplify 192 bands，including 188 polymorphic bands with a polymorphism ratio of 97.86%. The number of bands amplified by each primer ranged from 26 to 36，with an average of 32，and the number of polymorphic bands ranged from 26 to 36，with an average of 31.33 polymorphic bands amplified by each primer，and the polymorphic rate ranged from 90.00% to 100.00%. The polymorphic information content values（PIC）ranged from 0.9052 to 0.9527， with a mean of 0.9319. The Shannon’s information index（I）ranged from 0.2459 to 0.3878，the Nei’s gene diversity（H'） ranged from 0.1476 to 0.2412，the observed number of alleles（Na）ranged from 1.9000 to 2.0000，the number of effective alleles（Ne）ranged from 1.2324 to 1.3700. There were significant differences in polymorphic bands amplified by 6 SCoT primers. The number of germplasms that could be distinguished by single primer was 49-63，with an average of 58. According to the results of cluster analysis，the genetic similarity coefficients（GS）of 63 germplasm resources ranged from 0.5781 to 0.9115，with a mean of 0.7496. At the similarity coefficient of 0.7100，the tested materials were divided into five groups:group A included two germplasm resources from Guangxi；group B included a germplasm resource from Guangxi；group C inclu-ded 4 germplasm resources from Yunnan；group D included all germplasm resources from Hainan and Guangdong；group E included the remaining 50 germplasm resources. 5 SCoT primers（SCoT32，SCoT66， SCoT68，SCoT73，SCoT74）were further screened out to construct the DNA fingerprinting of 63 germplasm resources and each resource had specific DNA fingerprint code in the database.【Conclusion】The 63 germplasm resources are rich in genetic diversity，and there is a certain correlation between the kinship of the germplasm and the geographical area；the six SCoT primers screened are of high polymorphism，which can provide reference for the future studies on evaluation，genetic diversity analysis and fingerprinting on B. ramiflora Lour. germplasm resources. |
| |
| Keywords: | |
|
| 点击此处可从《南方农业学报》浏览原始摘要信息 |
|
点击此处可从《南方农业学报》下载全文 |
