| 鲤A-FABP基因的克隆及其表达谱研究 |
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| 引用本文： | 晁珊珊,刘茂琪,马美琳,张倡珲,字洪标,贺庆华,林亚秋.鲤A-FABP基因的克隆及其表达谱研究[J].西北农业学报,2014,23(6):43-47. |
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| 作者姓名： | 晁珊珊 刘茂琪 马美琳 张倡珲 字洪标 贺庆华 林亚秋 |
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| 作者单位： | （西南民族大学 生命科学与技术学院，成都 610041） |
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| 基金项目： | 西南民族大学国家级大学生创新创业训练计划（201310656012）。 |
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| 摘 要： | 为探索A-FABP基因表达与鲤肌内脂肪质量分数的关联,利用RT-PCR方法克隆鲤(Cyprinus carpio)脂肪细胞型脂肪酸结合蛋白(Adipocyte fatty acid binding protein,A-FABP)基因,同时利用荧光定量PCR技术分别检测该基因在鲤不同组织和不同发育阶段的表达差异,获得其组织和时序表达谱,并将基因表达水平与肌内脂肪质量分数进行关联分析。结果获得鲤A-FABP基因开放阅读框(ORF)序列405bp,编码134个氨基酸,与多鳞白甲鱼、斑马鱼、大西洋鲑和青鳉等的氨基酸同源性关系较近(氨基酸相似性为70%~91%),与人、猪、田鼠和牦牛等哺乳动物的氨基酸同源性关系较远(氨基酸相似性为51%~58%);组织表达谱分析结果显示,A-FABP基因可在鲤的心脏、肌肉、脂肪、肝脏、脑、脾脏、肾脏和鳃中表达,并且在脂肪组织的表达量显著高于其他组织(P0.05)。发育表达分析结果显示,较小体质量试验鱼(50g)的A-FABP基因表达水平显著高于较大体质量试验鱼(120~130g和500g)(P0.05),并且其表达与背最长肌的肌内脂肪质量分数呈显著负相关。
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| 关 键 词： | 鲤 A-FABP 克隆 表达 |
Cloning and Expression Profiles of Adipocyte-type Fatty Acid-binding Protein Gene in Cyprinus carpio |
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| CHAO Shanshan,LIU Maoqi,MA Meilin,ZHANG Changhui,ZI Hongbiao,HE Qinghua and LIN Yaqiu.Cloning and Expression Profiles of Adipocyte-type Fatty Acid-binding Protein Gene in Cyprinus carpio[J].Acta Agriculturae Boreali-occidentalis Sinica,2014,23(6):43-47. |
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| Authors: | CHAO Shanshan LIU Maoqi MA Meilin ZHANG Changhui ZI Hongbiao HE Qinghua and LIN Yaqiu |
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| Abstract: | To investigate the correlation between the expression of the A-FABP gene and the intramuscular fat content of the carp (Cyprinus carpio), the carp A-FABP gene was cloned by RT-PCR, the tissue and temporal expression profilesof A-FABP in carp were analyzed using fluorescence quantitative PCR, and the correlation between A-FABP expression level and the intramuscular fat content were analyzed. The results showed that carp A-FABP gene had an ORF species showed that, the sequence similarities were higher (70%-91%) in fishes (Onychostoma macrolepis, Danio rerio, Salmo salar, and Oryzias latipes) than those (51%-58%) in mammals (Homo sapiens, Sus scrofa, Microtus ochrogaster and Bos grunniens). The expression of A-FABP was observed in heart, muscle, fat, liver, brain, spleen, kidney and gill tissues, and the expression of A-FABP in fat tissue was significantly higher than other tissues studied (P<0.05). The expression of A-FABP gene in muscle carp with body weight of 50 g was markedly higher than those of 120-130 g and 500 g (P<0.05), however, its expression showed significant negative correlation with intramuscular fat content of muscle. of 405 bp, encoding a polypeptide of 134 amino acids. Amino acid sequences alignment of the carp A-FABP with A-FABPs from other |
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| Keywords: | Cyprinus carpio A-FABP Clone Expression |
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