The value of molecular expression of KIT and KIT ligand analysed using real‐time polymerase chain reaction and immunohistochemistry as a prognostic indicator for canine cutaneous mast cell tumours |
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| Authors: | T A Costa Casagrande L M de Oliveira Barros H Fukumasu B Cogliati L M Chaible M L Z Dagli J M Matera |
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| Affiliation: | 1. Biotechnology Master Program, Positivo University, Curitiba, Paraná, Brazil;2. Department of Surgery, School of Veterinary Medicine and Animal Science, University of S?o Paulo, S?o Paulo, Brazil;3. Department of Basic Sciences, School of Animal Science and Food Engineering, University of S?o Paulo, S?o Paulo, Brazil;4. Department of Pathology, School of Veterinary Medicine and Animal Science, University of S?o Paulo, S?o Paulo, Brazil |
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| Abstract: | This study investigated the correlation between KIT gene expression determined by immunohistochemistry and real‐time polymerase chain reaction (RT‐PCR) and the rate of tumour recurrence and tumour‐related deaths in dogs affected with mast cell tumour (MCT). Kaplan–Meier curves were constructed to compare tumour recurrence and tumour‐related death between patients. The log‐rank test was used to check for significant differences between curves. KIT‐I, KIT‐II and KIT‐III staining patterns were observed in 9 (11.11%), 50 (61.73%) and 22 (27.16%) tumours, respectively. Tumour recurrence rates and tumour‐related deaths were not associated with KIT staining patterns (P = 0278, P > 0.05), KIT (P = 0.289, P > 0.05) or KIT ligand (P = 0.106, P > 0.05) gene expression. Despite the lack of association between KIT staining pattern and patient survival time, the results suggest a correlation between aberrant KIT localization and increased proliferative activity of MCTs. RT‐PCR seems to be a sensible method for quantitative detection of KIT gene expression in canine MCT, although expressions levels are not correlated with prognosis. |
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| Keywords: | c‐kit immunohistochemistry mastocitoma survival time |
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