| 基于荧光光谱和二维相关分析技术探究硝磺草酮对玉米的生理效应 |
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| 引用本文： | 高倩,陈盼盼,王毅.基于荧光光谱和二维相关分析技术探究硝磺草酮对玉米的生理效应[J].农药学学报,2022,24(5):1248-1258. |
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| 作者姓名： | 高倩 陈盼盼 王毅 |
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| 作者单位： | 1.安徽农业大学 资源与环境学院，合肥 230036 |
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| 基金项目： | 安徽省自然科学基金优青项目 (2208085Y07)；国家自然科学基金 (32072464). |
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| 摘 要： | 为明确硝磺草酮胁迫对玉米的生理效应及其在玉米田使用的安全性，采用紫外可见吸收光谱、同步荧光光谱、三维荧光光谱和二维相关分析 (2D-COS) 技术，研究了不同浓度硝磺草酮对玉米叶片中叶绿素及类胡萝卜素含量，根、茎、叶鲜重和根系分泌物荧光物质的影响。结果表明:当硝磺草酮质量浓度为5和10 mg/L时，胁迫10及14 d后，玉米叶片中叶绿素和类胡萝卜素含量以及根、茎、叶鲜重均显著降低。同步荧光光谱、三维荧光光谱和2D-COS技术分析揭示，玉米根系分泌物中类蛋白和类腐殖质的荧光强度发生了显著变化，并且随着硝磺草酮浓度升高、胁迫时间延长，上述变化越明显；但其225~235 nm处的类蛋白荧光峰强度在胁迫7、10和14 d时反而增强，结合2D-COS分析发现，280~325 nm处的类蛋白荧光基团先与硝磺草酮进行结合。研究表明，硝磺草酮质量浓度高于5 mg/L，胁迫10 d后对玉米的正常生长会产生不利影响，研究结果可为评价硝磺草酮在玉米田使用的安全性提供理论依据。
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| 关 键 词： | 玉米 硝磺草酮 光谱技术 二维相关分析 生理效应 荧光强度 |
| 收稿时间： | 2022-07-22 |
Physiological effects of maize with mesotrione using fluorescence spectroscopy and two-dimensional?correlation?analysis technique |
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| Affiliation: | 1.College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | To clarify the physiological effect of mesotrione stress on maize and its use safety in a maize field, the effects of different concentrations of mesotrione on chlorophyll and carotenoid contents in leaves, fresh weights of roots, stems, and leaves, and fluorescence substances in root exudates of maize were studied using UV visible absorption spectrum, synchronous fluorescence spectrum, three-dimensional fluorescence spectrum, and two-dimensional correlation analysis (2D-COS) technique. The results showed that the chlorophyll and carotenoid contents in leaves and the fresh weights of roots, stems, and leaves of maize were significantly decreased after 10 and 14 d of stress with 5 and 10 mg/L mesotrione. The analysis of synchronous fluorescence spectrum, three-dimensional fluorescence spectrum, and 2D-COS technology revealed that the fluorescence intensity of protein-like and humus-like substances in the root exudates of maize had changed significantly, and the above changes were more notable with the increase of the concentration of mesotrione and the prolongation of the stress time. However, the intensity of the protein-like fluorescent peak at 225-235 nm was enhanced at 7, 10, and 14 d of stress. Combined with 2D-COS analysis, it was found that the protein-like fluorescent group at 280-325 nm exhibits preference binding to mesotrione. The results showed that when the concentration of mesotrione is higher than 5 mg/L, the normal growth of maize would be adversely affected after 10 d of stress. The research results can provide a theoretical basis for evaluating the safety of mesotrione on maize. |
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