| 枯草芽孢杆菌防治玉米纹枯病的初步研究 |
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| 引用本文： | 毛腾霄,叶华智,秦玉花. 枯草芽孢杆菌防治玉米纹枯病的初步研究[J]. 中国农学通报, 2016, 32(5): 44-48. DOI: 10.11924/j.issn.1000-6850.casb15090023 |
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| 作者姓名： | 毛腾霄 叶华智 秦玉花 |
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| 作者单位： | 成都市食品药品检验研究院,四川农业大学农学院,成都中医药大学基础医学院 |
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| 摘 要： | 玉米纹枯病(Rhizoctonia solani)的防治生产上主要依靠井冈霉素。为了从高效、安全、环保角度寻找井冈霉素的替代药剂,本研究将从玉米植株茎基部叶鞘分离得到的枯草芽孢杆菌菌株(Bacillus subtilis BS-8D)制成菌悬液,喷施于玉米幼苗和成株期基部叶鞘,并作了菌株抗利福平突变株的定殖能力测定以及室内平板抗生作用测定。结果表明,BS-8D菌株在温室内对玉米纹枯病有较好的防效。抗利福平标记突变株BS-8Drif定殖测定表明,菌株在玉米根际具有较好的定殖能力,施用10多天后仍能保持较多数量;菌株能随种子的萌发向地上和地下部分转移,在幼苗茎基部和根部定殖。室内PDA平板抗生作用测定表明该菌株还对玉米大斑病菌(Exsero turcicum)、玉米小斑病菌(Bipolaris maydis)、玉米新月弯孢菌(Curvularia lunata)有拮抗作用。BS-8D菌株对玉米纹枯病的防治实验表明,该菌株有进一步研究的价值和开发应用潜力。
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| 关 键 词： | 灰色模型GM ( 1 灰色模型GM ( 1 1) 蔬菜产量预测 都市型蔬菜产业 上海 |
| 收稿时间： | 2015-09-07 |
| 修稿时间： | 2015-10-19 |
Study on Biocontrol of Maize Sheath Blight (Rhizoctonia solani) with Bacillus subtilis Strain |
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| Abstract: | Jinggangmycin has been approved for controlling the maize sheath blight (caused by Rhizoctonia solani). In order to find a substitute that was safe, efficient and environmental friendly to Jinggangmycin, bacterial suspension of Bacillus subtilis BS-8D which was isolated from sheath of corn plants was used to spray on seedlings and basal part of stem at adult stage. Resistance of BS-8D to rifampicin (BS-8DRif) was tested for its ability to colonize plant roots in soil condition, and screened for its antibiosis in dual culture with other pathogenic fungus. The results showed that BS-8D could control corn sheath blight effectively in greenhouse. BS-8DRif showed that the strain could colonize, propagate and remain high populations for more than ten days in the rhizosphere of corn, and could be transported along with the elongating root and transmitted to stems of maize continuously. By dual culture on PDA medium, BS-8D showed strong ability against Exsero turcicum, Bipolaris maydism and Curvularia lunata. The control test showed BS-8D had potential for further research and development. |
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| Keywords: | Bacillus subtilis antagonistic bacterium maize sheath blight biocontrol field trials |
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