| 欧洲千里光CYCLOIDEA(CYC)类SvRAY1基因的克隆及功能分析 |
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| 引用本文： | 陈柯俐,朴春兰,郝燕敏,冯丽君,周佳圆,栾思楠,刘乐乐,李菲菲,袁思明,崔敏龙.欧洲千里光CYCLOIDEA(CYC)类SvRAY1基因的克隆及功能分析[J].浙江农林大学学报,2021,38(6):1153-1160. |
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| 作者姓名： | 陈柯俐 朴春兰 郝燕敏 冯丽君 周佳圆 栾思楠 刘乐乐 李菲菲 袁思明 崔敏龙 |
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| 作者单位： | 1.浙江农林大学 林业与生物技术学院，浙江 杭州 3113002.浙江农林大学 园艺科学学院，浙江 杭州 311300 |
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| 基金项目： | 浙江农林大学引进人才项目(2018FR004) |
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| 摘 要： | 目的 揭示菊科Asteraceae欧洲千里光Senecio vulgaris CYC2类RAY1基因过表达使舌状花发生不同程度变宽现象的机制，进一步探究其产生原因。 方法 克隆欧洲千里光SvRAY1基因，利用生物信息学、实时荧光定量PCR反应(qRT-PCR)、超表达载体构建、扫描电镜观察、转基因植株形态学观察与统计等方法与技术，进一步进行SvRAY1基因功能分析。 结果 qRT-PCR反应显示:SvRAY1基因主要在欧洲千里光舌状花及筒状花中表达，且生殖发育第S3和S4阶段舌状花中表达量最高；形态学观察表明转基因欧洲千里光SvRAY1超表达植株的舌状花比野生型长度较短、显著变宽。扫描电镜观察舌状花腹侧表皮细胞大小与形状，宽度显著变宽的株系中显示远轴端细胞排列紧密且细胞分裂旺盛，中轴端细胞形状由边缘弯曲变为平滑，细胞长度变短且分裂旺盛。 结论 欧洲千里光舌状花发育过程中，SvRAY1基因可能促进细胞横向分裂，进而舌状花细胞形态和排列发生不同程度的变化引起舌状花变宽。图6表2参28
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| 关 键 词： | 欧洲千里光 CYCLOIDEA(CYC)类基因 SvRAY1 舌状花发育 |
| 收稿时间： | 2020-12-28 |
Cloning and functional analysis of CYCLOIDEA(CYC)-like SvRAY1 gene from Senecio vulgaris |
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| CHEN Keli,PIAO Chunlan,HAO Yanmin,FENG Lijun,ZHOU Jiayuan,LUAN Sinan,LIU Lele,LI Feifei,YUAN Siming,CUI Minlong.Cloning and functional analysis of CYCLOIDEA(CYC)-like SvRAY1 gene from Senecio vulgaris[J].Journal of Zhejiang A&F University,2021,38(6):1153-1160. |
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| Authors: | CHEN Keli PIAO Chunlan HAO Yanmin FENG Lijun ZHOU Jiayuan LUAN Sinan LIU Lele LI Feifei YUAN Siming CUI Minlong |
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| Affiliation: | 1.College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China2.College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China |
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| Abstract: | Objective The aim of this study is to reveal the mechanism by which overexpression of RAY1 gene (CYC2-like genes) in Senecio vulgaris (Asteraceae) causes ray florets to broaden in varying degrees, and to further explore the underlying causes. Method SvRAY1 gene was cloned and analyzed by bioinformatics, qRT-PCR, construction of overexpression vector, SEM(scanning electron microscopy), and morphological observation and statistics of transgenic plants. Result qRT-PCR reaction showed that SvRAY1 gene was mainly expressed in ray florets and disc florets, with the highest expression level in the third and fourth stages of ray floret. Morphological observation showed that the ray floret of SvRAY1 overexpression plant was significantly shorter and wider than the wild type. The epidermal cells on the ventral side of the ray florets were observed by SEM and it was found that the width of the line was significantly wider than that of the wild type. The cells at the distal end were tightly arranged, small and divided vigorously. Cell division along the central axis was more vigorous than that of the wild type and the shape of cells changed from curved to smooth. Conclusion During the development of S. vulgaris, SvRAY1 gene may promote cell division, and change the morphology and arrangement of ray florets cells in varying degrees, resulting in the widening of the ray floret. Ch, 6 fig. 2 tab. 28 ref.] |
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