Inosine‐5'‐monophosphate is a candidate agent to resolve rigor mortis of skeletal muscle |
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| Authors: | Masanori Matsuishi Mariko Tsuji Megumi Yamaguchi Natsumi Kitamura Sachi Tanaka Yukinobu Nakamura Akihiro Okitani |
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| Affiliation: | 1. Department of Food Science and Technology, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan;2. Japan Meat Science and Technology Institute, Shibuya, Tokyo, Japan |
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| Abstract: | The object of the present study was to reveal the action of inosine‐5'‐monophosphate (IMP) toward myofibrils in postmortem muscles. IMP solubilized isolated actomyosin within a narrow range of KCl concentration, 0.19‐0.20 mol/L, because of the dissociation of actomyosin into actin and myosin, but it did not solubilize the proteins in myofibrils with 0.2 mol/L KCl. However, IMP could solubilize both proteins in myofibrils with 0.2 mol/L KCl in the presence of 1 m mol/L pyrophosphate or 1.0–3.3 m mol/L adenosine‐5'‐diphosphate (ADP). Thus, we presumed that pyrophosphate and ADP released thin filaments composed of actin, and thick filaments composed of myosin from restraints of myofibrils, and then both filaments were solubilized through the IMP‐induced dissociation of actomyosin. Thus, we concluded that IMP is a candidate agent to resolve rigor mortis because of its ability to break the association between thick and thin filaments. |
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| Keywords: | ADP IMP myofibrils pyrophosphate rigor mortis resolution |
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