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Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development |
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| Authors: | Akiyoshi Egashira Nobuhiko Yamauchi Md Rashedul Islam Kazuki Yamagami Asami Tanaka Hikaru Suyama El‐Sharawy Mohamed El‐Sayed Shoji Tabata Takashi Kuramoto |
| Affiliation: | 1. Kuramoto Women's Clinic, Fukuoka, Japan;2. Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan;3. Department of Genetics and Animal Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh;4. Animal Production Department, Kafrelsheikh University, Kafrelsheikh, Egypt |
| Abstract: | This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1‐ and 2‐cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4‐cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2‐ to 4‐cell arrested embryos and reduce the developmental competence to blastocyst. |
| Keywords: | Kid multinucleated blastomere parthenogenetic embryos siRNA |
