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| 诱导型启动子Rd29A 引导的植物表达载体的构建 | |
| 引用本文： | 卓仁英,孙宗修. 诱导型启动子Rd29A 引导的植物表达载体的构建[J]. 浙江农林大学学报, 2004, 21(3): 243-246 |
| 作者姓名： | 卓仁英 孙宗修 |
| 作者单位： | 1.中国林业科学研究院 亚热带林业研究所, 浙江 富阳 311400;2.中国水稻研究所 国家水稻生物学重点实验室, 浙江 杭州 310006 |
| 基金项目： | 浙江省自然科学基金重点资助项目(ZA0204) |
| 摘 要： | 全球20 %的耕地和近半数的灌溉土地都受到不同程度的盐害威胁, 抗盐育种具有重要意义。针对常规育种周期长等明显的局限性, 利用抗盐转基因可以迅速获得抗盐材料, 明显缩短育种周期。利用PCR 技术克隆获得Rd29A 这一干旱、盐碱等逆境诱导表达的特异性启动子, 与叶绿体转运肽和甜菜碱醛脱氢酶基因构建pBinRdMCS 重组质粒, 用于林木转化。通过克隆Rd29A 启动子构建逆境诱导的高效表达载体, 可对林木抗盐转基因育种提供理论指导。图5 参8 |
| 关 键 词： | 林木 抗盐 Rd29A 启动子 BADH |
| 收稿时间： | 2003-10-20 |
Rd29A gene cloning and plant expression vector constructed |
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| ZHUO Ren-ying,SUN Zong-xiu. Rd29A gene cloning and plant expression vector constructed[J]. Journal of Zhejiang A&F University, 2004, 21(3): 243-246 | |
| Authors: | ZHUO Ren-ying SUN Zong-xiu |
| Affiliation: | 1.The Research Institute of Subtropical Forest , CAF, Fuyang 311400 , Zhejiang , China;2.The State Key Laboratory of Rice Biology , Chinese National Rice Research Institute , Hangzhou 310006, Zhejiang , China |
| Abstract: | It was global problem that effected of saline on forest and agriculture soil , about 20 % plowlands and half of irrigated soils had been affected by saline .It's important to speeded salt-tolerance breeding course . Transgenic breeding can fasted the salt-tolerance pace , and shorten breeding cycle significantly .So we designed the primer and cloning Rd 29A promoter by PCR , constucted pBinRdMCS plasmid that BADH gene can be induced by salt and drought , then it was transit to chloroplast .The results analysis by PCR and restriction show that the plasmid include aim gene .The plasmid will be used for tree transgenic breeding .[ Ch , 5 fig .8 ref .] |
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