| 原儿茶酸或绿原酸与抗菌药联用对鱼源链球菌的抑菌效果 |
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| 引用本文： | 柴贝贝,姜维佳,王莉贞,胡梅,赵雨川,吴永继,司红彬. 原儿茶酸或绿原酸与抗菌药联用对鱼源链球菌的抑菌效果[J]. 南方农业学报, 2018, 49(3): 580-585. DOI: 10.3969/j.issn.2095-1191.2018.03.25 |
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| 作者姓名： | 柴贝贝 姜维佳 王莉贞 胡梅 赵雨川 吴永继 司红彬 |
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| 作者单位： | 广西大学 动物科学技术学院,南宁,530004 |
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| 基金项目： | 广西重大科技攻关项目国家自然科学基金项目南宁市科学研究技术开发计划项目 |
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| 摘 要： | [目的]探讨原儿茶酸或绿原酸与抗菌药联用对鱼源链球菌的抑菌效果,为鱼源链球菌病的综合防治提供参考依据.[方法]采用微量二倍稀释法测定原儿茶酸、绿原酸及各种抗菌药对鱼源链球菌的最小抑菌浓度(MIC),并以1/2MIC的原儿茶酸或绿原酸分别联合抗菌药诱导鱼源链球菌进行传代培养,然后使用微量棋盘稀释法测定原儿茶酸或绿原酸与抗菌药联合作用后的部分抑菌浓度指数(FICI).[结果]原儿茶酸和绿原酸对鱼源链球菌的MIC分别为2500和6000 μg/mL,以1250 μg/mL的原儿茶酸和3000 μg/mL的绿原酸分别与痢菌净、阿米卡星、粘菌素、磺胺间甲氧嘧啶钠、克林沙星和磷霉素等抗菌药联合诱导鱼源链球菌传代后,除磺胺间甲氧嘧啶钠外,其他5种抗菌药对传代获得菌株的MIC明显降低.原儿茶酸与抗菌药配伍后的FICI分别为0.500、0.750、0.516、1.125、0.140和0.516,绿原酸与抗菌药配伍后的FICI分别为0.501、0.625、0.501、>2.000、0.140和0.501.依据FICI的判定标准可知,原儿茶酸与痢菌净和克林沙星配伍呈协同效应,与阿米卡星、粘菌素和磷霉素配伍呈相加效应,与磺胺间甲氧嘧啶钠配伍表现为无关效应;绿原酸与阿米卡星和克林沙星配伍呈协同效应,与痢菌净、粘菌素和磷霉素配伍呈相加效应,与磺胺间甲氧嘧啶钠配伍则呈拮抗效应.[结论]原儿茶酸或绿原酸与抗菌药配伍后能增强大部分抗菌药对鱼源链球菌的抑菌活性,呈协同或相加作用,可在水产养殖生产中推广应用.
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| 关 键 词： | 鱼源链球菌 原儿茶酸 绿原酸 联用 最小抑菌浓度(MIC) 部分抑菌浓度指数(FICI) |
Antibacterial effects of protocatechuic acid and chlorogenic acid combined with antibiotics against fish-source Streptococcus |
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| CHAI Bei-bei,JIANG Wei-jia,WANG Li-zhen,HU Mei,ZHAO Yu-chuan,WU Yong-ji,SI Hong-bin. Antibacterial effects of protocatechuic acid and chlorogenic acid combined with antibiotics against fish-source Streptococcus[J]. Journal of Southern Agriculture, 2018, 49(3): 580-585. DOI: 10.3969/j.issn.2095-1191.2018.03.25 |
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| Authors: | CHAI Bei-bei JIANG Wei-jia WANG Li-zhen HU Mei ZHAO Yu-chuan WU Yong-ji SI Hong-bin |
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| Abstract: | [Objective]The antibacterial effects of protocatechuic acid and chlorogenic acid combined with antibiotic on fish-source Streptococcus were studied to provide reference for the comprehensive prevention and treatment of fish-source Streptococcus disease.[Method]The minimum inhibitory concentrations(MIC)of protocatechuic acid,chlorogenic acid and antibiotics against fish-source Streptococcus were tested through double microdilution method,and fish-source Streptococcus were induced to subculture by inhibitory concentration(1/2MIC)of protocatechuic acid or chlorogenic acid combined with antibiotics respectively.Then the fractional inhibitory concentration indexes(FICI)of protocatechuic acid or chlorogenic acid combined with antibiotics respectively were determined by microdilution checkerboard method.[Re-sult]The MICs of protocatechuic acid and chlorogenic acid against fish-source Streptococcus were 2500 μg/mL and 6000 μg/mL.After fish-source Streptococcus being induced to subculture by 1250 μg/mL protocatechuic acid and 3000 μg/mL chlorogenic acid respectively combined with mequindox,amikacin,colistin,sulfamonomethoxine,clinafloxacin,and fosfomycin,the MICs of the antibiotics greattly decreased except sulfamonomethoxine. When protocatechuic acid was combined with the antibiotics mentioned above,the FICIs were 0.500,0.750,0.516,1.125,0.140 and 0.516 respective-ly.When chlorogenic acid was combined with the antibiotics mentioned above,the FICIs were 0.501,0.625,0.501,>2.000, 0.140 and 0.501.According to the criteria for FICI,there existed synergistic effects when protocatechuic acid was com-bined with mequindox and clinafloxacin;there existed additive effects when protocatechuic acid was combined with ami-kacin,colistin and fosfomycin,but there were indifferent effects when protocatechuic acid was mixed with sulfamono-methoxine.There were synergistic effects when chlorogenic acid was combined with amikacin and clinafloxacin;there existed additive effects after chlorogenic acid was mixed with mequindox,colistin and fosfomycin,but there were indif-ferent effects when chlorogenic acid was combined with sulfamonomethoxine.[Conclusion]The combination of protocate-chuic acid or chlorogenic acid with antibiotics can enhance the antibacterial activity of most tested antibiotics against fish-source Streptococcus and presents synergistic effects or additive effects.Therefore,it can be promoted in aquaculture. |
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