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| 注射液中非法添加地塞米松磷酸钠UPLC-PDA检查方法的建立 | |
| 引用本文： | 韩春晓,李阳,李洪伟,陈晨,高红倩. 注射液中非法添加地塞米松磷酸钠UPLC-PDA检查方法的建立[J]. 中国畜牧兽医, 2016, 43(10): 2748-2755. DOI: 10.16431/j.cnki.1671-7236.2016.10.035 |
| 作者姓名： | 韩春晓 李阳 李洪伟 陈晨 高红倩 |
| 作者单位： | 1. 辽宁省兽药饲料畜产品质量安全检测中心, 沈阳 110016; 2. 沈阳药科大学中药学院, 沈阳 110016 |
| 基金项目： | 畜禽产品安全生产监控和检测技术研究与示范（2014BAD13B05） |
| 摘 要： | 本试验建立了鱼腥草、柴胡、维生素C、盐酸林可霉素、黄芪多糖等注射液中非法添加地塞米松磷酸钠的超高效液相色谱检查方法。采用Waters ACQUITY UPLC® BEH C18色谱柱（2.1 mm×50 mm，1.7 μm），以0.75%三乙胺溶液（磷酸调节pH 3.0）-甲醇-乙腈（50：45：5）为流动相，流速0.3 mL/min，二极管阵列检测器，波长采集范围为190~400 nm，记录色谱图波长242 nm，柱温25℃，进样量10 μL。地塞米松磷酸钠质量浓度在10.0~200.0 mg/L内峰面积呈良好线性关系（R2=0.9999），在鱼腥草、柴胡、维生素C、盐酸林可霉素、黄芪多糖等注射液中地塞米松磷酸钠的平均回收率和相对标准偏差（RSD）分别为97.54%和1.14%、101.59%和0.19%、100.92%和0.92%、99.82%和0.73%、100.08%和0.62%。本方法简单、快速、灵敏、可靠，适用于鱼腥草等5种注射液中非法添加地塞米松磷钠的检查。 |
| 关 键 词： | 超高效液相法 鱼腥草注射液 柴胡注射液 维生素C注射液 盐酸林可霉素注射液 黄芪多糖注射液 地塞米松磷酸钠 |
| 收稿时间： | 2016-02-15 |
Establishment of Detection Methods for Dexamethasone Sodium Phosphate in Injections by UPLC-PDA |
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| HAN Chun-xiao,LI Yang,LI Hong-wei,CHEN Chen,GAO Hong-qian. Establishment of Detection Methods for Dexamethasone Sodium Phosphate in Injections by UPLC-PDA[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(10): 2748-2755. DOI: 10.16431/j.cnki.1671-7236.2016.10.035 | |
| Authors: | HAN Chun-xiao LI Yang LI Hong-wei CHEN Chen GAO Hong-qian |
| Affiliation: | 1. Liaoning Province Animal Feed Quality and Safety of Veterinary Testing Centers, Shenyang 110016, China; 2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China |
| Abstract: | A detection method of dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection,and asragulus polysacharin injection was established by UPLC-PDA.The Waters ACQUITY UPLC® BEH C18 column(2.1 mm×50 mm,1.7 μm) was used,the mobile phase consisted of 0.75% triethylamine phosphatic liquor/solution (pH 3.0)-methyl alcohol-acetonitrile(50:45:5),the flow rate was 0.3 mL/min,the column temperature had remained 25℃,photodiode array detector wavelength range was 190 to 400 nm,the recorded wavelength was 242 nm,the injection volume was 10 μL.The linear range of dexamethasone sodium phosphatel was 10.0 to 200.0 mg/L (R2=0.9999),the mean recovery and RSD for dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection and asragulus polysacharin injection were 97.54% and 1.14%,101.59% and 0.19%,100.92% and 0.92%,99.82% and 0.73%,100.08% and 0.62%,respectively.This method was accurate,easy and fast,it was suitable for detection of dexamethasone sodium phosphatein houttuynia injection and other four kinds of injections. |
| Keywords: | UPLC houttuynia injection bupleurum injection vitamin C injection lincomycin hydrochloride injection asragulus polysacharin injection dexamethasone sodium phosphat |
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