| 肉桂游离酚提取物改善非酒精性肝细胞脂肪变性的活性研究 |
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| 引用本文： | 李会鹏,庞道睿,刘瑶瑶,邢东旭,钟赛意,黎尔纳,廖森泰,邹宇晓.肉桂游离酚提取物改善非酒精性肝细胞脂肪变性的活性研究[J].南方农业学报,2022,53(3):879-890. |
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| 作者姓名： | 李会鹏 庞道睿 刘瑶瑶 邢东旭 钟赛意 黎尔纳 廖森泰 邹宇晓 |
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| 作者单位： | 1. 广东海洋大学食品科技学院, 广东湛江 524088;2. 广东省农业科学院蚕业与农产品加工研究所/农业农村部功能食品重点实验室/广东省农产品加工重点实验室/岭南现代农业科学与技术广东省实验室茂名分中心, 广东广州 510610 |
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| 基金项目： | 广东省农业科学院新兴学科团队建设项目;广东省重点领域研发计划项目;广东省现代农业产业共性关键技术研发创新团队建设项目;广东省实验室茂名分中心自主科研项目 |
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| 摘 要： | 【目的】分析云野肉桂中不同存在形式酚类物质的含量，并探究其不同存在形式酚类提取物对非酒精性脂肪肝（Non-alcoholic fatty liver disease，NAFLD）的影响，为热带亚热带植物肉桂资源的深加工及相关功能性食品的开发和利用提供理论依据。【方法】用福林酚法及硼氢化钠—四氯对苯醌法分析肉桂中不同存在形式的酚类物质及黄酮类物质的含量；体外以游离脂肪酸诱导剂（油酸钠∶棕榈酸=2∶1）诱导HepG2细胞为模型，分析不同浓度的肉桂游离酚/结合酚提取物对NAFLD细胞模型脂滴形成及甘油三酯（TG）含量的影响；体内以高脂饮食结合链脲佐菌素诱导的NAFLD小鼠为模型，探究体外筛选的强活性酚类提取物对小鼠血脂及肝脏中TG和游离脂肪酸含量的影响。【结果】肉桂多酚主要以游离酚（55.99±0.45 mg GAE/g）的形式存在，含有一定比例的结合酚（3.21±0.12 mg GAE/g），其中黄酮类物质（46.49±4.75 mg CE/g）是肉桂的主要酚类物质之一。云野肉桂游离酚提取物可有效降低NAFLD细胞模型胞内TG的水平。与模型组相比，经中和高剂量的肉桂游离酚提取物作用后，细胞内TG含量显著降低（P<0.05，下同），分别降低17.07%和26.83%，而结合酚提取物作用后细胞内TG水平与模型组相比无显著差异（P>0.05）。动物试验结果表明，高剂量的游离酚提取物干预后，不仅可以显著降低小鼠血清中TG、胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）含量，还能显著降低小鼠肝脏中TG和游离脂肪酸含量，分别降低42.21%和22.22%。液相色谱串联质谱（LC-MS/MS）分析结果表明，肉桂游离酚提取物主要由原花青素C1、原花青素B2、原花青素A2、原花青素B3和原花青素B1等物质组成。【结论】肉桂游离酚提取物对NAFLD可能具有较好的改善作用，且原花青素类物质可能是发挥改善肝细胞脂肪变性作用的主要物质基础。
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| 关 键 词： | 肉桂提取物 多酚 脂肪变性 NAFLD细胞模型 小鼠 |
| 收稿时间： | 2022-01-14 |
Effects of Cinnamon cassia free phenolic extract on nonalcoholic hepatocyte steatosis |
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| LI Hui-peng,PANG Dao-rui,LIU Yao-yao,XING Dong-xu,ZHONG Sai-yi,LI Er-na,LIAO Sen-tai,ZOU Yu-xiao.Effects of Cinnamon cassia free phenolic extract on nonalcoholic hepatocyte steatosis[J].Journal of Southern Agriculture,2022,53(3):879-890. |
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| Authors: | LI Hui-peng PANG Dao-rui LIU Yao-yao XING Dong-xu ZHONG Sai-yi LI Er-na LIAO Sen-tai ZOU Yu-xiao |
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| Affiliation: | 1. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China;2. Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Food, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing/Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, Guangdong 510610, China |
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| Abstract: | 【Objective】The contents of different forms of phenols of Cinnamon cassia and the effects of different forms of phenolic extracts on non-alcoholic fatty liver disease(NAFLD)were studied in order to provide a theoretical basis for the processing of cinnamon resources and the development of functional foods of cinnamon.【Method】The contents of phenols and flavonoids in cinnamon were analyzed by the Folin phenol method and the sodium borohydride/chloranil based assay(SBC),respectively. HepG2 cells induced by sodium oleate and palmitic acid(2:1)were used as an in vitro model to analyze the regulation effects of different concentrations of free or bound phenolic extracts of C. cassia on the formation of intracellular lipid droplets. The change of triglyceride(TG)content of different groups was also determined. Compared with the bound phenolic extract,the free polyphenol extract of cinnamon showed a better effect on steatosis. Furthermore,mice fed on a high sugar and high fat diet in combination with streptozotocin(STZ)was used as to model the in vivo effects of the phenolic extracts on serum and liver lipids.【Result】The results suggested that polyphenols(55.99±0.45 mg GAE/g)existed in cinnamon extracts mainly in free form. Notably,some bound polyphenols(3.21±0.12 mg GAE/g) were also detected. The flavonoids(46.49±4.75 mg CE/g)were the main component of cinnamon polyphenols. The free phenolic extract of the Yunye cultivar of cinnamon can effectively reduce the TG level in vitro. The extracts of medium and high dose groups significantly decreased the TG level by 17.07% and 26.83% relative to that of the model group,respectively(P<0.05,the same below). There was no significant difference in intracellular TG level between the model group and the bound phenol extract group(P>0.05). The results of animal experiments showed that the contents of TG,total cholesterol(TC)and low-density lipoprotein cholesterol(LDL-C)in the serum of mice decreased significantly after the intervention of high dose free phenol extract. High dose cinnamon free phenol extract also significantly reduced the contents of TG and FFA in the liver of mice,which decreased by 42.21% and 22.22%,respectively. The results of LC-MS/MS showed that procyanidins C1,B2,A2,B3 and B1 may be the main flavonoid compounds of the cinnamon free phenolic extract.【Conclusion】These results reveal that cinnamon free phenolic extract may have a good effect on NAFLD,and procyanidins may be the main compounds effective against the steatosis of liver cells |
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