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Enrichment of Adult Artemia Biomass and Squid Mantle Muscle,Dosidicus gigas,with Different Ascorbic Acid (L‐Ascorbyl‐2‐Monophosphate‐Na/Ca) Concentrations
Authors:Miguel E Marentes‐Montes  Jesús T Ponce Palafox  Sergio Castillo‐Vargasmachuca  Irma E Martínez‐Rodríguez  Jorge Castro Mejia  Manuel García‐Ulloa  Alireza Seidavi
Affiliation:1. Estudiante del Posgrado de Ciencias Biológicas y Agropecuarias (CBAP), Universidad Autónoma de Nayarit, Xalisco, Nayarit 63780, México;2. Universidad Autónoma de Nayarit, CENITT‐Posgrado CBAP, Escuela Nacional de Ingeniería Pesquera, Lab. Bioingeniería Costera, Centro Multidisciplinario de Bahía de Banderas, Nayarit 6155, México;3. Centro de Investigación en Alimentación y Desarrollo‐Unidad Mazatlán en Acuicultura y Medio Ambiente, Mazatlán, Sinaloa 82000, México;4. Universidad Autónoma Metropolitana‐Xochimilco, Depto. El Hombre y su Ambiente, Laboratorio de Producción de Alimento Vivo 04960, México;5. Department of Animal Science, Rasht Branch, Islamic Azad University, PO Box 41857‐43999, Rasht, Iran
Abstract:L‐ascorbyl‐2‐monophosphate‐Na/Ca (AMP‐Na/Ca) was used as a vitamin C source to investigate its ascorbic acid (L‐AA) enrichment and retention in boosted Artemia biomass (AB) and squid mantle muscle (SM). Different doses of AMP‐Na/Ca (500, 1000, and 1500 AMP‐Na/Ca mg/kg) were gradually dissolved into the culture tanks at time 0 (T0) and at each hour until Hour 6 (T6). Samples of AB and SM were taken for AMP‐Na/Ca and L‐AA analysis at T0, T1, T2, T3, T4, T5, T6, T12, and T24. There were no significant differences (P > 0.05) among the AB groups at T1. The T6 enrichment analysis for AB resulted in significant differences (P < 0.05) in the AMP‐Na/Ca content for the 1500 mg/kg treatment, in which the initial concentration (0.001 ± 0.002 mg/kg) increased by more than 16‐fold. For all AB enrichment treatments, the AMP‐Na/Ca content demonstrated a decrease (32–11%) for the T6, T12 and T24 analysis. The T1 analysis for SM at the higher AMP‐Na/Ca enrichment concentration registered 30 mg/kg of L‐AA and decreased (27.6%) at T6. This study demonstrated that AB and SM can be boosted with AMP‐Na/Ca.
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