Abstract:

On the basis of foot and mouth disease virus (FMDV) inactivated bivalen
t(O type, AsiaⅠ type) vaccines(called conventional FMDV vaccines blew ) using 206 adjuvant (France SEPPIC company), a double adjuvant inactivated bivalent FMDV vaccines was designed, it possessed stronger delayed release effect than conventional FMDV vaccines. According to the lymphocytes proliferation reaction (MTT), LPELISA and challenge protection experiments,the lymphocytes stimulation indices (SI=1.235±0.060) of double adjuvant vaccines group was higher than conventional FMDV vaccines group (SI=1.115±0.035) and control group (SI=1.010±0.045); compared with conventional FMDV vaccines group and control group, the celluar celluar immunity level of double adjuvant vaccines group was significant than conventional one (P＜0.05) and significant stronger than control one (P＜0.01). However, as full dose immunity, antibody titer of conventional FMDV vaccines and double adjuvant vaccines group had little difference, 1/3 dose and 1/9 dose groups were especially lower than above two groups for less antigen and strong delayed release effect. The results of challenge protection efficiency showed that conventional adjuvant vaccines group was a little higher than the FMDV vaccines group, the former O strain was 11.84 PD50 and AsiaⅠ strain was 9.0PD50; the the latter O strain and AsiaⅠ strain all were 9.0 PD50. The results suggested that double adjuvant vaccines could induce better cellular immunity, possessed stronger delayed release effect and supported better challenge protection efficiency than conventional FMDV vaccines.

Key words: FMDV inactivated vaccines; adjuvant; cellular immunity; humoral immunity; challenge protection efficiency