家养观赏地图鱼肺炎克雷伯氏菌、维氏气单胞菌与黏质沙雷氏菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2015.01.034

Abstract

Abstract: The study was aimed to determine the cause of death of a domesticated ornamental Astronotus ocellatus and screen sensitive drugs via isolation and identification of related bacteria.First, the bacteria were isolated and purified by conventional methods for observation of bacterial morphology.Then, identification and drug resistance analysis were performed through methods of pathogenicity test on mice, bacteria biochemical identification, 16S rDNA sequencing and analysis, drug susceptibility test and resistance gene detection.Three Gram-negative short bacilli were isolated and identified, according to their morphological characteristics and physical and chemical characteristics as well as the results of 16S rDNA sequencing and phylogenetic analysis, as Klebslella pneumoniae, Aeromonas veronii and Serratia marcescens.Among them, Klebsiella pneumoniae had strong pathogenicity.The three bacteria were sensitive to lomefloxacin, ofloxacin, amikacin, kanamycin, but had a strong resistance to amoxicillin, florfenicol, clindamycin, metronidazole.A mixed infection of Klebsiella pneumoniae, Aeromonas veronii and Serratia marcescens was the cause of death of domesticated ornamental Astronotus ocellatus.

Key words: Astronotus ocellatus; Klebsiella pneumoniae; Aeromonas veronii; Serratia marcescens; isolation and identification; drug susceptibility characteristics

CLC Number:

S941

CAO Ying-ying, DU Hong-ming, BAI An-bin, WANG Li-bo, MA Ling, TANG Cheng-ming, WU Jian-min. Isolation, Identification and Drug Resistance of Klebsiella pneumoniae, Aeromonas veronii and Serratia marcescens Isolated from Domesticated Ornamental Astronotus ocellatus[J]. , 2015, 42(1): 215-223.

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Isolation, Identification and Drug Resistance of Klebsiella pneumoniae, Aeromonas veronii and Serratia marcescens Isolated from Domesticated Ornamental Astronotus ocellatus

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