丙泊酚静脉麻醉与安氟醚吸入麻醉在巴马小型猪体外循环中的比较研究

›› 2013, Vol. 40 ›› Issue (4): 238-241.

丙泊酚静脉麻醉与安氟醚吸入麻醉在巴马小型猪体外循环中的比较研究

陈克研, 刁玉刚, 金强, 王洪乾, 姚国泉, 张铁铮

沈阳军区总医院麻醉科,辽宁沈阳 110016

收稿日期:2012-09-20 出版日期:2013-04-20 发布日期:2013-04-19
通讯作者: 张铁铮(1961-),男,硕士生导师,主要从事临床麻醉学研究。E-mail:tzzhang@hotmail.com E-mail:tzzhang@hotmail.com
作者简介:陈克研(1983-),男,内蒙古人,博士,主要从事基础医学研究。
基金资助:
国家自然青年科学基金项目(31201758)。

Comparative of Propofol Intravenous Anesthesia and Enflurane Inhalation Anesthesia in Cardiopulmonary Bypass of Bama Minipigs

CHEN Ke-yan, DIAO Yu-gang, JIN Qiang, WANG Hong-qian, YAO Guo-quan, ZHANG Tie-zheng

Department of Anesthesiology,General Hospital of Shenyang Military Area Command,Shenyang 110016,China

Received:2012-09-20 Online:2013-04-20 Published:2013-04-19

摘要/Abstract

摘要： 为探讨丙泊酚静脉麻醉与安氟醚吸入麻醉在巴马小型猪体外循环(CPB)中的麻醉效果,本研究选用巴马小型猪10头,平均分成2组,分别进行丙泊酚静脉麻醉和安氟醚吸入麻醉;开胸后进行全血肝素化处理,分别于升主动脉、上腔静脉和下腔静脉插管,连接体外循环机进行CPB;观察并记录麻醉诱导前(T0)、麻醉诱导后(T1)、CPB前即刻(T2)、降温至30 ℃(T3)、阻断主动脉前即刻(T4)、阻断主动脉后4 min(T5)、开始复温即刻(T6)、停CPB即刻(T7)、关胸后即刻(T8)及手术结束拔管后10 min(T9)10个时间点各组试验猪的生命体征、鼻温(NT)、心率(HR)、平均动脉压(MAP)、pH及血氧饱和度(SPO₂)指标。结果2组试验猪于CPB实施前后的基本情况如体重、手术时间和麻醉时间等指标均无显著性差异(P＞0.05);安氟醚组恢复自主呼吸的时间显著短于丙泊酚组(P＜0.05);与T0和T1相比,各组试验猪在CPB进行时其NT、HR和MAP值均显著降低(P＜0.05);但各组间及组内SPO₂和pH差异不显著(P＞0.05)。由此可见,丙泊酚静脉麻醉与安氟醚吸入麻醉均可用于巴马小型猪CPB中的麻醉。选用丙泊酚时,应根据手术过程中试验动物的反应情况适当调整用量;而安氟醚麻醉过程相对平稳,麻醉效果好,术后苏醒快,适合情况复杂且时间较长的手术。

关键词: 安氟醚; 丙泊酚; 巴马小型猪; 体外循环

Abstract: The assay was aimed to compare of propofol intravenous anesthesia and enflurane inhalation anesthesia in cardiopulmonary bypass of Bama Minipigs. 10 Bama Minipigs, the average into two groups were propofol intravenous anesthesia and enflurane inhalation anesthesia, and in preoperative, operative and postoperative the anesthesia monitoring. After openning breast, whole blood were treated with heparin, then the ascending aorta, superior vena cava and inferior vena cava were intubated respectively, and connected to a cardiopulmonary bypass machine line the CPB. The vital signs, nose temperature (NT) , heart rate (HR), mean arterial pressure (MAP), pH and blood oxygen saturation(SPO₂) of each experimental pigs were observe and recorded within before introdution of anesthesia (T0), after induction of anesthesia (T1), immediately before CPB (T2), cooling to 30 ℃ (T3), blocking the aorta former immediate (T4), blocking the aorta 4 min (T5), start thawing immediate (T6), stop CPB immediate (T7), closed chest immediately after (T8) and drawing tube operation over 10 min (T9). Two groups of weight number of pigs, operation time and anesthesia time all had no significant difference (P＞0.05). Different fluorine ether group restore spontaneous breathing a short time in propofol group (P＜0.05). Compared with the basic value(T0 and T1), the NT, HR and MAP value of the experiment pigs were significantly lower (P＜0.05). But each rooms and in the group SPO₂ value and pH were not significant different (P＞0.05). Propofol intravenous anesthesia during the operation should be based on the experimental animals of the reaction conditions appropriate adjustments propofol pump into the concentration; and different fluorine ether inhaled anesthesia during the procedure of smooth, anaeesthetic effect was good, postoperative came quickly, suitable for complicated cases, and the time was long operation.

Key words: enflurane; propofol; Bama Minipigs; cardiopulmonary bypass

中图分类号:

R332

陈克研, 刁玉刚, 金强, 王洪乾, 姚国泉, 张铁铮. 丙泊酚静脉麻醉与安氟醚吸入麻醉在巴马小型猪体外循环中的比较研究[J]. , 2013, 40(4): 238-241.

CHEN Ke-yan, DIAO Yu-gang, JIN Qiang, WANG Hong-qian, YAO Guo-quan, ZHANG Tie-zheng. Comparative of Propofol Intravenous Anesthesia and Enflurane Inhalation Anesthesia in Cardiopulmonary Bypass of Bama Minipigs[J]. , 2013, 40(4): 238-241.

参考文献

1 王凯,黄丽洁,陈华,等.小型猪的麻醉及气管插管技术[J].中国比较医学杂志,2011,28(1):49~51.
2 王爱德.广西巴马小型猪的选育研究[J].中国比较医学杂志,2004,14(3):160.
3 王朝军,于桂萍.猪安氟醚吸入麻醉的研究[J].中国兽医杂志,1996,14(4):29~32.
4 宋丹丹,张铁铮,姚婧,等.大鼠无血预充体外循环伴心脏停跳模型的建立[J].中国体外循环杂志,2009,4(7):232~235.
5 张永奎,韩辉,乐涵波,等.抑肽酶与氨甲环酸在体外循环中血液保护的对比研究[J].中国航天医药杂志,2002,1:21~23.
6 金士翱,林桂芳.临床麻醉学[M].北京:中国医药科技出版社,1992.
7 范宏刚,胡魁,卢德章,等. 小型猪麻醉研究进展[J]. 中国畜牧兽医, 2009, 36(11): 178~179.
8 Anderson L.A phylogenetic study of the origin of the domestic pig estimated from the near-complete mtDNA genome[J].J Mol Evol,2001,52:302~308.
9 Delange F,Dieleman J M,Jungwirth B,et al.Effects of cardiopulmonary bypass on neurocognitive performance and cytokine release in old and diabetic rats[J].Br J Anaesth,2007,99(2):177~183.
10 Gibbon J H.Application of a mechanical heart and lung apparatus to cardiac surgery[J].Minn Med,1954,37:171.
11 Hubert M B,Salazkin L,Desjardins J,et al.Cardiopulmonary bypass surgery in swine:A research model[J].J Exp Anim Sci,2003,43:135~149.
12 Jayaraman S,Davies W,Schlachta C M.Getting started with robotics in general surgery with cholecystectomy:The Canadian experience[J].Can J Surg,2009,52(5):374~378.
13 Lee L,Alloosh M,Saxena R,et al.Nutritional model of steatohepatitis and metabolic syndrome in the Ossabaw miniature swine[J].Hepatology,2009,50(1):56~67.
14 Qu K X,Wu G S,Gou X,et al.Genetic differentiations between randomly and selectively bred pig populations in Yunnan,China[J].Zoological Research,2011,32(3):255~261.
15 She J Q,Wang M,Zhu D M,et al.Effect of ganglioside on synaptic plasticity of hippocampus in lead-exposed rats in vivo[J].Brain Res,2005,1060(1~2):162~169.
16 Silva G V,Fernandes M R,Madonna R,et al.Comparative healing response after sirolimus-and paclitaxel-eluting stent implantation in a pig model of restenosis[J].Catheter Cardiovasc Iuterv,2009,73(6):801~808.

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