›› 2009, Vol. 36 ›› Issue (4): 69-72.

• 生物技术 • Previous Articles     Next Articles

The Establishment and Preliminary Application of Detection Method of Mixed Infection both Classical Swine Fever and Porcine Circovirus Type 2

LI Jin-tao1, BI Bao-liang1, LIU Xu-chuan1, LI Yan-bing1, GUI Yi1, LI Zuo-sheng2, ZHANG Yi-fang1   

  1. (1.College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;2.Medical Research Institute of Chengdu Army Garrion Center for Disease Control and Prevention, Kunming 650032, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-04-20 Published:2009-04-20
  • Contact: ZHANG Yi-fang

Abstract: According to the published nucleotide sequence of CSFV and PCV2 in GenBank, one pair of specific primers of nucleotide sequence of CSFV and PCV2 were designed respectively by NCBI/Primer-BLAST and synthesized. The PCR and RT-PCR method to PCV2 and CSFV was established, and 19 clinical samples collected from eight pig farms in Kunming from April to August in 2008 were detected to CSFV and PCV2. The results showed that, four samples from three farms were positive CSFV, the infection rate of positive CSFV was 21.05 percent; two samples from two farms were positive PCV2, the infection rate of positive PCV2 was 10.53 percent. CSFV and PCV2 mixed infection rate was 10.53 percent. The established detection method of mixed infection both CSFV and PCV2 was specific, and can be used for detecting CSFV and PCV2 mixed infection and epidemiological investigation, and can also provided scientific basis for prevention and control of CSFV and PCV2.

Key words: classical swine fever; porcine circovirus type 2; mixed infection; detection

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