| 玉米穗腐病样品中层出镰刀菌的分离与鉴定 |
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| 引用本文：卢维宏,黄思良,陶爱丽,武爱波,王春梅,黎起秦.玉米穗腐病样品中层出镰刀菌的分离与鉴定.植物保护学报,2011,38(3):233-239 |
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| 中文摘要:为了解豫西南玉米穗腐病的病原菌,于2009年9月在河南省南阳市6个县区采集玉米穗腐病样品,进行病原菌的分离与单孢纯化。依据致病性、形态及分子检测结果进行病原菌鉴定。研究结果表明,分离得到的105株镰刀菌中,层出镰刀菌Fusarium proliferatum为70株,占分离镰刀菌的66.7%,属优势种群。在基于核糖体基因内转录间隔区(rDNA-ITS)与翻译延长因子1α基因(tef)序列构建的系统发育树中,3株代表菌株Fp1、Fp2和Fp3分别与GenBank登记的层出镰刀菌在自举值89%和91%水平相聚同一群。层出镰刀菌的生长温度为13~34 ℃, 最适温度为28 ℃; 在pH 5.0和6.0各有1个生长高峰;碳源对该菌营养生长的影响较为稳定,而氮源对其营养生长影响的变幅较大。 |
| 中文关键词:玉米 层出镰刀菌 致病性 鉴定 |
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| Isolation and characterization of Fusarium proliferatum from maize ear rot samples |
| Author Name | Affiliation | E-mail | | Lu Weihong | College of Life Sciences and Technology, Nanyang Normal University, Nanyang 473061, Henan Province, China
College of Agriculture, Guangxi University, Nanning 530005, Guangxi Zhuang Autonomous Region, China | | | Huang Siliang | College of Life Sciences and Technology, Nanyang Normal University, Nanyang 473061, Henan Province, China | silianghuang@126.com | | Tao Aili | College of Life Sciences and Technology, Nanyang Normal University, Nanyang 473061, Henan Province, China
College of Agriculture, Guangxi University, Nanning 530005, Guangxi Zhuang Autonomous Region, China | | | Wu Aibo | College of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai 200240, China | | | Wang Chunmei | College of Life Sciences and Technology, Nanyang Normal University, Nanyang 473061, Henan Province, China | | | Li Qiqin | College of Agriculture, Guangxi University, Nanning 530005, Guangxi Zhuang Autonomous Region, China | |
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| Abstract:To know the pathogens causing maize ear rot in southwestern Henan, the maize ear rot samples were collected from 6 counties in Nanyang City in September, 2009. Pathogens were isolated from the samples and purified with single spore isolation. The pathogens were identified based on results of pathogenicity tests, morphological observations and molecular analyses. Seventy isolates were identified as members of Fusarium proliferatum, contributing about 66.7% of the Fusarium isolates obtained and indicating that F.proliferatum was a predominant Fusarium species causing maize ear rot in Nanyang. Two phylogenetic trees were established based on rDNA-ITS (internal transcribed spacer) and tef (translation elongation factor 1α gene) sequences, respectively. Three representative isolates Fp1, Fp2 and Fp3 clustered with GenBank-derived F.proliferatum isolates at 89% bootstrap level for rDNA-ITS sequences, and 91% for tef gene sequences. F.proliferatum could grow at temperatures of 13-34 ℃ with 28 ℃ as the optimum. Growth peaks occurred at pH 5.0 and pH 6.0, respectively. Vegetative growth of F.proliferatum was found to be significantly influenced by nitrogen sources compared to carbon sources. |
| keywords:maize Fusarium proliferatum pathogenicity identification |
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