pH胁迫对中国明对虾抗氧化系统酶活力及基因表达的影响

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pH胁迫对中国明对虾抗氧化系统酶活力及基因表达的影响
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作者单位:1. 上海海洋大学 水产与生命学院, 上海 201306;2. 中国水产科学研究院 黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室, 山东 青岛266071
作者简介:王芸 (1981), 女, 博士研究生, 主要从事环境毒理学研究. Tel: 0532-85826690; E-mail: wangyun088@tom.com
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基金项目:国家自然科学基金项目(40706052); 农业部公益性行业专项(200803012, 201103034); 国家虾产业技术体系项目(CARS-47); 国家863计划项目(2010AA10A401); 科技部农业科技成果转化资金项目(2010GB23260589).

Effects of pH stress on antioxidant system enzyme activities and gene expression of Fenneropenaeus chinensis

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1.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture; Yellow Sea Fisheries Research Institute, Chinese Academy of Fi

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摘要:

pH7.0于胁迫后测定鳃、肝胰腺、肌肉和血淋巴总抗氧化活力(CAT), pH上述指标受到抑制。对虾肝胰腺和肌肉, 基因表达随胁迫时间增加呈下降趋势。高对虾鳃抗氧化系统酶活力及基因表达水平达峰时间较其他而低对虾肝胰腺抗氧化系统酶活力比其他组织变化快。实验结果表明对中国明对虾抗氧化系统酶活力及相关基因表达有一定的诱导效果则抑制其抗氧化系统功能中国明对虾鳃和肝胰腺分别对高、低

Abstract:

, is an important mariculture species in China. Recently deteriorated pond environment due to intensive culture caused increased incidence of stress-induced diseases. It is well known that environmental stress can induce oxidative stress. However, there is little information about the effects of pH changes on antioxidant system of Chinese shrimp. In this study, Chinese shrimps were exposed to water of pH7.0 and 9.0 for 148 h and pH8.2 was set as control. T (T-AOC), anti-superoxide anion activity, catalase (CAT) activity and the expression ) gene were analyzed in gill, hepatopancreas, muscle and haemocytes of Chinese shrimp after h exposure. The T-AOC, anti-superoxide anion activity, CAT activity and the gene expression of in different tissues increased within 1214 h of pH stress. However, they were inhibited in the extending periods of pH stress time. The gene expression in heaptopancreas and muscle gradually increased, but decreased in gill and haemocytes with pH stress time prolonging. The enzyme activities and gene expression of antioxidant system in gills first reached its highest level in pH 9.0 group, faster than other tissues in 9.0 group. And the antioxidant enzyme activities in hepatopancreas in pH 7.0 group changed faster than in other tissues. The results indicate that the antioxidant enzyme activities and gene expression of Chinese shrimp were induced by short-term pH stress which is the response to oxidative stress, while they were inhibited with pH stress time prolonging. It was shown that long time neutral and alkaline-induced oxidative stress probably caused the antioxidant defense system injury in Chinese shrimp. Gills and hepatopancreas could be the sensitive tissues to high and low pH stress, respectively.

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王芸,李健,李吉涛,何玉英,常志强,刘德月. pH胁迫对中国明对虾抗氧化系统酶活力及基因表达的影响[J].中国水产科学,2011,18(3):556-564
WANG Yun, LI Jian, LI Jitao, HE Yuying, CHANG Zhiqiang, LIU Deyue. Effects of pH stress on antioxidant system enzyme activities and gene expression of Fenneropenaeus chinensis[J]. Journal of Fishery Sciences of China,2011,18(3):556-564

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在线发布日期: 2011-05-09
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