三倍体九孔鲍的育种和养成技术研究
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福建海洋研究所生物室

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福建省科技厅科研项目


Seed breeding and culturing of triploid abalone Haliotis diversicolor aquatilis
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Biology group,Fujian Institute of Oceanology, Xiamen 361012,China

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    摘要:

    当50%九孔鲍受精卵出现第一极体后,以6-DMAP为诱导剂,用不同的浓度和不同的持续时间抑制第二极体的释放诱导三倍体;并进行了生产性苗种培育和养成试验.结果表明,6-DMAP的诱导浓度为300μmol@dm-3、诱导持续时间为10min时,其胚胎的三倍体率为90%以上,并且胚胎的孵化率高达85%,幼体的畸形率较低,为50%~55%;经过7个月培育,试验组养成鲍的三倍体率为65%以上;试验组养成鲍的平均壳长比对照组增长10%,平均体重比对照组增重30%.与二倍体鲍相比较,三倍体鲍在壳长增长和体重增重等方面,显示了显著的优势[t>t0.01(58)].

    Abstract:

    When 50% of zygotes of Haliotis diversicolor aquatilis develop polar body . , with 6- DMAP as derivat ive , various concentration and dif ferent treatment time are used to induce triplo id by inhibiting releasing of the second polar body ò ; the experiment was carried out for productive seed breeding and culturing. Results show that when 6- DMAP is at the concentration of 300 Lmol#dm - 3 , and treatment time is 10min, the triploid rate of embryo is above 90%; In addition, embryonic hatching rate can reach as high as 85%, with larval deformity rate as low as 50%- 55% . After 7 months of cultivation, the rate of triploid of the culture abalone in test group is above 65%. On-the-spot acceptance check by experts proves that the average length of the shell of the culture abalone in test group grew 10% longer than that of control group. The average weight of the culture abalone in test group grew 30% weightier than that of the control group. Compared with diploid control group, triploid abalone displayed notable advantages in the growth of such aspect s as shell length and body weight ( t> t 0. 01( 58) ) .

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严正凛.三倍体九孔鲍的育种和养成技术研究[J].水产学报,2002,26(1):

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历史
  • 收稿日期:2014-04-11
  • 最后修改日期:2014-04-11
  • 录用日期:2014-04-12
  • 在线发布日期: 2014-04-12
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