不同温度下形成的深海菌膜对厚壳贻贝幼虫变态的影响
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上海海洋大学

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Q958

基金项目:

国家自然科学(41876159,41476131,41606147);国家重点研发计划(2018YFC0310600);上海高校水产高峰学科建设项目。


Li T W. Marine Biology[M]. Beijing: China Ocean Press, 2013 (in Chinese).
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Shanghai Ocean University

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    摘要:

    为探讨深海细菌生物被膜对温度的适应性及对厚壳贻贝(Mytilus coruscus)幼虫变态的影响,分别在4、18、25、37 ℃条件下培养生物被膜,调查温度对细菌密度、膜厚和胞外产物等生物学特性的影响,以及被膜对幼虫变态发育的影响。结果表明:四个温度条件下产生的生物被膜均可有效促进幼虫变态发育。其中,深海菌株Virgibacillus sp. 1在18 ℃时生物被膜的变态诱导活性最高(35%),且α胞外多糖含量较高,诱导活性与温度和细菌密度均显著相关;同时温度与其细菌密度和膜厚均显著相关(P<0.05)。2株深海假交替单胞菌诱导活性与细菌密度均显著相关,温度仅影响Pseudoalteromonas sp. 33细菌密度。上述结果表明3株深海细菌均有很好的温度适应性,且都能形成生物被膜;温度变化导致生物被膜的生物学特性改变,最终影响其对幼虫的诱导效果。

    Abstract:

    Effects of water temperature (4, 18, 25 and 37 °C) on deep-sea bacterial biofilms and subsequent effect on metamorphosis of larvae of the mussel Mytilus coruscus were studied. The confocal laser scanning microscopy technique was used to clarify the characteristics of biofilms including biofilm thickness and the distribution of extracellular polymeric substances. The biofilms formed by the deep-sea bacteria could promote the metamorphosis of larvae under four temperature conditions. Virgibacillus sp.1 showed the highest inductive activity (35%), with high α-extracellular polysaccharide secretion. The Virgibacillus sp.1 biofilm inducing activities on larval metamorphosis were correlated to seawater temperature and bacterial density. Temperature was significantly correlated with bacterial density, induction activity and biofilm thickness (P<0.05).The densities of bacteria in the biofilms of the two Pseudoalteromonas strains were significantly correlated with the induced activity. This present study shows that deep-sea bacteria exhibited good potential on temperature change to form the biofilms, and impacted larval metamorphosis.

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梁箫,杨丽婷,彭莉华,魏玉利,方家松,杨金龙.不同温度下形成的深海菌膜对厚壳贻贝幼虫变态的影响[J].水产学报,2020,44(10):1728~1741

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  • 收稿日期:2019-12-10
  • 最后修改日期:2020-03-25
  • 录用日期:2020-04-14
  • 在线发布日期: 2020-11-06
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