Differentially expressed genes(DEGs) associated with phytohormone pathway were analyzed by transcriptome sequencing in potato tubers stored at low temperature for 5d, which from potato insensitive cultivar(‘Huashu3’) and sensitive one(‘Zhongshu5’) to cold-induced sweetening(CIS). Six DEGs closely associated with sugar metabolism were screened from DEGs in phytohormone pathway related with phytohormone synthesis and signal transduction through bioinformatics analysis. And then the effects of these 6 DEGs on potato CIS were analyzed. The results were as follows: KOox2 and GA3ox1 are the key enzyme genes for gibberellin(GA) biosynthesis in potato tubers. GA could stimulate the activities of amylase and invertase, and increase the accumulation of reducing sugar in tuber. Thus GA was a positive regulator of potato CIS. ANT and AIL6 belong to AP2 transcription factor family involved in ethylene response. BRI1 and BKI1 are two transcription factors in brassinosteroid(BR) signaling pathway, and they could interact and then antagonize the activity of 14-3-3 protein. These 4 transcription factors ANT, AIL6, BRI1 and BKI1 affected the reducing sugar production through negative regulation of invertase gene expression, thus were negative regulators for potato CIS.