玉米干旱诱导表达基因ZmBTF3b的克隆与表达分析

doi:10.3864/j.issn.0578-1752.2010.16.002

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (16): 3280-3287 .doi: 10.3864/j.issn.0578-1752.2010.16.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles Next Articles

Isolation and Expression Analysis of a Drought-Induced Gene ZmBTF3b in Maize (Zea mays L.)

ZHANG Zhong-bao, ZHANG Deng-feng, LI Hui-yong, LIU Ying-hui, SHI Yun-su, SONG Yan-chun, WANG Tian-yu, LI Yu

(中国农业科学院作物科学研究所)

Received:2010-02-09 Revised:2010-03-19 Online:2010-08-15 Published:2010-08-15
Contact: LI Yu,WANG Tian-yu

Abstract

Abstract:

【Objective】 ZmBTF3b gene was cloned and used to analyze the expression profiles under abiotic stresses, providing a foundation for investigating mechanisms of molecular regulation in stress tolerance in maize. 【Method】 The promoter of ZmBTF3b was analyzed via bioinformatics. Transcriptional activation activity of ZmBTF3b was conducted using yeast one-hybrid system. Expression profiles of ZmBTF3b in different tissues of maize and in response to abiotic stresses were assayed via real-time quantitative PCR (qRT-PCR). 【Result】 The cloned ZmBTF3b gene encoded a putative transcription factor of 169 amino acids with a conserved NAC (nascent polypeptide-associated complex) domain. Transcriptional activity analysis showed that ZmBTF3b might be a functional transcription factor. Real-time quantitative PCR (qRT-PCR) analysis revealed that ZmBTF3b was highly expressed in silks, ears and immature embryos and was up-regulated under dehydration or PEG treatment while was down-regulated under cold, NaCl, ABA or SA. 【Conclusion】 ZmBTF3b might have the transcriptional activation activity and play an essential role in response to abiotic stresses.

Key words: Zea mays, ZmBTF3b, drought stress, expression profiles, transcriptional activation

ZHANG Zhong-bao,ZHANG Deng-feng,LI Hui-yong,LIU Ying-hui,SHI Yun-su,SONG Yan-chun,WANG Tian-yu,LI Yu
. Isolation and Expression Analysis of a Drought-Induced Gene ZmBTF3b in Maize (Zea mays L.)
[J].Scientia Agricultura Sinica, 2010, 43(16): 3280-3287 .

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