| 摘要: |
| 为了用分子生物学方法对油桃品质进行分析，本研究利用RAPD技术，采用从200个10碱基随机引物筛选的22个引物对19个油桃品种的基因组DNA进行扩增，通过扩增180个位点谱带的聚类，分析供试油桃的系统发育，将供试油桃品种分为3类；并运用特殊谱带，建立了油桃的品种分子识别表，提出了重点保存的油桃种质有曙光、瑞光2号、五月火、艾米拉、红李光、阿姆肯。 |
| 关键词: 油桃 RAPD 系统发育 品种 分子识别表 种质保存 分子生物学 |
| DOI： |
| 分类号:S662.1 |
| 基金项目:武汉市晨光计划资助项目 (9850 0 30 74)，华中农业大学国家作物改良重点实验室资助项目，中国科学院方向资助项目 (KSCX2 - SW- 1 0 4 ) |
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| Analysis of Nectarine Cultivars Revealed by RAPD |
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Cheng Zhongping 1 2 Chen Zhiwei 2 Hu Chungen 3 Deng Xiuxin 3
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| Abstract: |
| To analyze nectarine cultivars from angle of molecular biology, genomic DNAs of 19 accessions of nectarine peach ( Amgdalus persica var. necturina ) were amplified by RAPD (Randomly Amplified Polymorphic DNA) with 22 primers selected from 200 arbitrary 10 decamer primers. Analyzing phylogenetic relationship through cluster of the bands of 180 amplified loci. The experimented accessions could be divided among three groups. Molecular checking index and conservation of key germplasm which included 6 cultivars named Shuguang, Ruiguang No.2, Mayfire, Aimila, Hongliguang, Armking were put forward according to special bands. |
| Key words: nectarine peach,RAPD,phylogenetic relationship,molecular checking index,conservation of key germplasm |
